Show simple item record

dc.contributor.authorZhang, Y
dc.contributor.authorGuillermier, C
dc.contributor.authorDe Raedt, T
dc.contributor.authorCox, AG
dc.contributor.authorMaertens, O
dc.contributor.authorYimlamai, D
dc.contributor.authorLun, M
dc.contributor.authorWhitney, A
dc.contributor.authorMaas, RL
dc.contributor.authorGoessling, W
dc.contributor.authorCichowski, K
dc.contributor.authorSteinhauser, ML
dc.date.accessioned2020-11-17T03:28:59Z
dc.date.available2020-11-17T03:28:59Z
dc.date.issued2020-08-21
dc.identifierpii: S2589-0042(20)30542-3
dc.identifier.citationZhang, Y., Guillermier, C., De Raedt, T., Cox, A. G., Maertens, O., Yimlamai, D., Lun, M., Whitney, A., Maas, R. L., Goessling, W., Cichowski, K. & Steinhauser, M. L. (2020). Imaging Mass Spectrometry Reveals Tumor Metabolic Heterogeneity. ISCIENCE, 23 (8), https://doi.org/10.1016/j.isci.2020.101355.
dc.identifier.issn2589-0042
dc.identifier.urihttp://hdl.handle.net/11343/251514
dc.description.abstractMalignant tumors exhibit high degrees of genomic heterogeneity at the cellular level, leading to the view that subpopulations of tumor cells drive growth and treatment resistance. To examine the degree to which tumors also exhibit metabolic heterogeneity at the level of individual cells, we employed multi-isotope imaging mass spectrometry (MIMS) to quantify utilization of stable isotopes of glucose and glutamine along with a label for cell division. Mouse models of melanoma and malignant peripheral nerve sheath tumors (MPNSTs) exhibited striking heterogeneity of substrate utilization, evident in both proliferating and non-proliferating cells. We identified a correlation between metabolic heterogeneity, proliferation, and therapeutic resistance. Heterogeneity in metabolic substrate usage as revealed by incorporation of glucose and glutamine tracers is thus a marker for tumor proliferation. Collectively, our data demonstrate that MIMS provides a powerful tool with which to dissect metabolic functions of individual cells within the native tumor environment.
dc.languageEnglish
dc.publisherCELL PRESS
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleImaging Mass Spectrometry Reveals Tumor Metabolic Heterogeneity
dc.typeJournal Article
dc.identifier.doi10.1016/j.isci.2020.101355
melbourne.affiliation.departmentBiochemistry and Molecular Biology
melbourne.source.titleiScience
melbourne.source.volume23
melbourne.source.issue8
dc.rights.licenseCC BY
melbourne.elementsid1458564
melbourne.contributor.authorCox, Andrew
dc.identifier.eissn2589-0042
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record