University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Doherty Institute
  • Doherty Institute - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Doherty Institute
  • Doherty Institute - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Intrahepatic CXCL10 is strongly associated with liver fibrosis in HIV-Hepatitis B co-infection

    Thumbnail
    Download
    published version (3.224Mb)

    Citations
    Altmetric
    Author
    Singh, KP; Zerbato, JM; Zhao, W; Braat, S; Deleage, C; Tennakoon, GS; Mason, H; Dantanarayana, A; Rhodes, A; Rhodes, JW; ...
    Date
    2020-09-01
    Source Title
    PLoS Pathogens
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Braat, Sabine; Lewin, Sharon; Audsley, Jennifer
    Affiliation
    Doherty Institute
    Melbourne School of Population and Global Health
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Singh, K. P., Zerbato, J. M., Zhao, W., Braat, S., Deleage, C., Tennakoon, G. S., Mason, H., Dantanarayana, A., Rhodes, A., Rhodes, J. W., Torresi, J., Harman, A. N., Revill, P. A., Crane, M., Estes, J. D., Avihingsanon, A., Lewin, S. R. & Audsley, J. (2020). Intrahepatic CXCL10 is strongly associated with liver fibrosis in HIV-Hepatitis B co-infection. PLOS PATHOGENS, 16 (9), https://doi.org/10.1371/journal.ppat.1008744.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/251563
    DOI
    10.1371/journal.ppat.1008744
    Abstract
    In HIV-hepatitis B virus (HBV) co-infection, adverse liver outcomes including liver fibrosis occur at higher frequency than in HBV-mono-infection, even following antiretroviral therapy (ART) that suppresses both HIV and HBV replication. To determine whether liver disease was associated with intrahepatic or circulating markers of inflammation or burden of HIV or HBV, liver biopsies and blood were collected from HIV-HBV co-infected individuals (n = 39) living in Bangkok, Thailand and naïve to ART. Transient elastography (TE) was performed. Intrahepatic and circulating markers of inflammation and microbial translocation were quantified by ELISA and bead arrays and HIV and HBV infection quantified by PCR. Liver fibrosis (measured by both transient elastography and liver biopsy) was statistically significantly associated with intrahepatic mRNA for CXCL10 and CXCR3 using linear and logistic regression analyses adjusted for CD4 T-cell count. There was no evidence of a relationship between liver fibrosis and circulating HBV DNA, qHBsAg, plasma HIV RNA or circulating cell-associated HIV RNA or DNA. Using immunohistochemistry of liver biopsies from this cohort, intrahepatic CXCL10 was detected in hepatocytes associated with inflammatory liver infiltrates in the portal tracts. In an in vitro model, we infected an HBV-infected hepatocyte cell line with HIV, followed by interferon-γ stimulation. HBV-infected cells lines produced significantly more CXCL10 than uninfected cells lines and this significantly increased in the presence of an increasing multiplicity of HIV infection. Conclusion: Enhanced production of CXCL10 following co-infection of hepatocytes with both HIV and HBV may contribute to accelerated liver disease in the setting of HIV-HBV co-infection.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [52443]
    • Melbourne School of Population and Global Health - Research Publications [5315]
    • Doherty Institute - Research Publications [373]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors