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dc.contributor.authorReijneveld, JF
dc.contributor.authorOcampo, TA
dc.contributor.authorShahine, A
dc.contributor.authorGully, BS
dc.contributor.authorVantourout, P
dc.contributor.authorHayday, AC
dc.contributor.authorRossjohn, J
dc.contributor.authorMoody, DB
dc.contributor.authorVan Rhijn, I
dc.date.accessioned2020-11-17T03:38:37Z
dc.date.available2020-11-17T03:38:37Z
dc.date.issued2020-09-15
dc.identifierpii: 2010545117
dc.identifier.citationReijneveld, J. F., Ocampo, T. A., Shahine, A., Gully, B. S., Vantourout, P., Hayday, A. C., Rossjohn, J., Moody, D. B. & Van Rhijn, I. (2020). Human gamma delta T cells recognize CD1b by two distinct mechanisms. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 117 (37), pp.22944-22952. https://doi.org/10.1073/pnas.2010545117.
dc.identifier.issn0027-8424
dc.identifier.urihttp://hdl.handle.net/11343/251567
dc.description.abstractγδ T cells form an abundant part of the human cellular immune system, where they respond to tissue damage, infection, and cancer. The spectrum of known molecular targets recognized by Vδ1-expressing γδ T cells is becoming increasingly diverse. Here we describe human γδ T cells that recognize CD1b, a lipid antigen-presenting molecule, which is inducibly expressed on monocytes and dendritic cells. Using CD1b tetramers to study multiple donors, we found that many CD1b-specific γδ T cells use Vδ1. Despite their common use of Vδ1, three CD1b-specific γδ T cell receptors (TCRs) showed clear differences in the surface of CD1b recognized, the requirement for lipid antigens, and corecognition of butryophilin-like proteins. Several Vγ segments were present among the CD1b-specific TCRs, but chain swap experiments demonstrated that CD1b specificity was mediated by the Vδ1 chain. One of the CD1b-specific Vδ1+ TCRs paired with Vγ4 and shows dual reactivity to CD1b and butyrophilin-like proteins. αβ TCRs typically recognize the peptide display platform of MHC proteins. In contrast, our results demonstrate the use of rearranged receptors to mediate diverse modes of recognition across the surface of CD1b in ways that do and do not require carried lipids.
dc.languageEnglish
dc.publisherNATL ACAD SCIENCES
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleHuman gamma delta T cells recognize CD1b by two distinct mechanisms
dc.typeJournal Article
dc.identifier.doi10.1073/pnas.2010545117
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titleProceedings of the National Academy of Sciences of USA
melbourne.source.volume117
melbourne.source.issue37
melbourne.source.pages22944-22952
dc.rights.licenseCC BY
melbourne.elementsid1464536
melbourne.contributor.authorRossjohn, Jamie
dc.identifier.eissn1091-6490
melbourne.accessrightsOpen Access


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