High frequency acoustic cell stimulation promotes exosome generation regulated by a calcium-dependent mechanism.
AuthorAmbattu, LA; Ramesan, S; Dekiwadia, C; Hanssen, E; Li, H; Yeo, LY
Source TitleCommunications Biology
Chemical and Biomolecular Engineering
Document TypeJournal Article
CitationsAmbattu, L. A., Ramesan, S., Dekiwadia, C., Hanssen, E., Li, H. & Yeo, L. Y. (2020). High frequency acoustic cell stimulation promotes exosome generation regulated by a calcium-dependent mechanism.. Communications Biology, 3 (1), https://doi.org/10.1038/s42003-020-01277-6.
Access StatusOpen Access
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536404
Exosomes are promising disease diagnostic markers and drug delivery vehicles, although their use in practice is limited by insufficient homogeneous quantities that can be produced. We reveal that exposing cells to high frequency acoustic irradiation stimulates their generation without detriment to cell viability by exploiting their innate membrane repair mechanism, wherein the enhanced recruitment of calcium ions from the extracellular milieu into the cells triggers an ESCRT pathway known to orchestrate exosomal production. Given the high post-irradiation cell viabilities (≈95%), we are able to recycle the cells through iterative irradiation and post-excitation incubation steps, which facilitate high throughput production of a homogeneous population of exosomes-a particular challenge for translating exosome therapy into clinical practice. In particular, we show that approximately eight- to ten-fold enrichment in the number of exosomes produced can be achieved with just 7 cycles over 280 mins, equivalent to a yield of around 1.7-2.1-fold/h.
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