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dc.contributor.authorAmbattu, LA
dc.contributor.authorRamesan, S
dc.contributor.authorDekiwadia, C
dc.contributor.authorHanssen, E
dc.contributor.authorLi, H
dc.contributor.authorYeo, LY
dc.date.accessioned2020-11-17T03:59:44Z
dc.date.available2020-11-17T03:59:44Z
dc.date.issued2020-10-05
dc.identifierpii: 10.1038/s42003-020-01277-6
dc.identifier.citationAmbattu, L. A., Ramesan, S., Dekiwadia, C., Hanssen, E., Li, H. & Yeo, L. Y. (2020). High frequency acoustic cell stimulation promotes exosome generation regulated by a calcium-dependent mechanism.. Communications Biology, 3 (1), https://doi.org/10.1038/s42003-020-01277-6.
dc.identifier.issn2399-3642
dc.identifier.urihttp://hdl.handle.net/11343/251665
dc.description.abstractExosomes are promising disease diagnostic markers and drug delivery vehicles, although their use in practice is limited by insufficient homogeneous quantities that can be produced. We reveal that exposing cells to high frequency acoustic irradiation stimulates their generation without detriment to cell viability by exploiting their innate membrane repair mechanism, wherein the enhanced recruitment of calcium ions from the extracellular milieu into the cells triggers an ESCRT pathway known to orchestrate exosomal production. Given the high post-irradiation cell viabilities (≈95%), we are able to recycle the cells through iterative irradiation and post-excitation incubation steps, which facilitate high throughput production of a homogeneous population of exosomes-a particular challenge for translating exosome therapy into clinical practice. In particular, we show that approximately eight- to ten-fold enrichment in the number of exosomes produced can be achieved with just 7 cycles over 280 mins, equivalent to a yield of around 1.7-2.1-fold/h.
dc.languageeng
dc.publisherNature Research
dc.titleHigh frequency acoustic cell stimulation promotes exosome generation regulated by a calcium-dependent mechanism.
dc.typeJournal Article
dc.identifier.doi10.1038/s42003-020-01277-6
melbourne.affiliation.departmentBio21
melbourne.affiliation.departmentChemical and Biomolecular Engineering
melbourne.source.titleCommunications Biology
melbourne.source.volume3
melbourne.source.issue1
melbourne.source.pages553-
dc.rights.licenseCC BY
melbourne.elementsid1467930
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536404
melbourne.contributor.authorHanssen, Eric
melbourne.contributor.authorRamesan, Shwathy
dc.identifier.eissn2399-3642
melbourne.accessrightsOpen Access


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