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dc.contributor.authorBrouwer, S
dc.contributor.authorBarnett, TC
dc.contributor.authorLy, D
dc.contributor.authorKasper, KJ
dc.contributor.authorDe Oliveira, DMP
dc.contributor.authorRivera-Hernandez, T
dc.contributor.authorCork, AJ
dc.contributor.authorMcIntyre, L
dc.contributor.authorJespersen, MG
dc.contributor.authorRichter, J
dc.contributor.authorSchulz, BL
dc.contributor.authorDougan, G
dc.contributor.authorNizet, V
dc.contributor.authorYuen, K-Y
dc.contributor.authorYou, Y
dc.contributor.authorMcCormick, JK
dc.contributor.authorSanderson-Smith, ML
dc.contributor.authorDavies, MR
dc.contributor.authorWalker, MJ
dc.date.accessioned2020-11-17T03:59:54Z
dc.date.available2020-11-17T03:59:54Z
dc.date.issued2020-10-06
dc.identifierpii: 10.1038/s41467-020-18700-5
dc.identifier.citationBrouwer, S., Barnett, T. C., Ly, D., Kasper, K. J., De Oliveira, D. M. P., Rivera-Hernandez, T., Cork, A. J., McIntyre, L., Jespersen, M. G., Richter, J., Schulz, B. L., Dougan, G., Nizet, V., Yuen, K. -Y., You, Y., McCormick, J. K., Sanderson-Smith, M. L., Davies, M. R. & Walker, M. J. (2020). Prophage exotoxins enhance colonization fitness in epidemic scarlet fever-causing Streptococcus pyogenes. NATURE COMMUNICATIONS, 11 (1), https://doi.org/10.1038/s41467-020-18700-5.
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/11343/251666
dc.description.abstractThe re-emergence of scarlet fever poses a new global public health threat. The capacity of North-East Asian serotype M12 (emm12) Streptococcus pyogenes (group A Streptococcus, GAS) to cause scarlet fever has been linked epidemiologically to the presence of novel prophages, including prophage ΦHKU.vir encoding the secreted superantigens SSA and SpeC and the DNase Spd1. Here, we report the molecular characterization of ΦHKU.vir-encoded exotoxins. We demonstrate that streptolysin O (SLO)-induced glutathione efflux from host cellular stores is a previously unappreciated GAS virulence mechanism that promotes SSA release and activity, representing the first description of a thiol-activated bacterial superantigen. Spd1 is required for resistance to neutrophil killing. Investigating single, double and triple isogenic knockout mutants of the ΦHKU.vir-encoded exotoxins, we find that SpeC and Spd1 act synergistically to facilitate nasopharyngeal colonization in a mouse model. These results offer insight into the pathogenesis of scarlet fever-causing GAS mediated by prophage ΦHKU.vir exotoxins.
dc.languageEnglish
dc.publisherNATURE RESEARCH
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleProphage exotoxins enhance colonization fitness in epidemic scarlet fever-causing Streptococcus pyogenes
dc.typeJournal Article
dc.identifier.doi10.1038/s41467-020-18700-5
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titleNature Communications
melbourne.source.volume11
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1467932
melbourne.contributor.authorDougan, Gordon
melbourne.contributor.authorDavies, Mark
dc.identifier.eissn2041-1723
melbourne.accessrightsOpen Access


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