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dc.contributor.authorAreeb, Z
dc.contributor.authorStuart, SF
dc.contributor.authorWest, AJ
dc.contributor.authorGomez, J
dc.contributor.authorNguyen, HPT
dc.contributor.authorParadiso, L
dc.contributor.authorZulkifli, A
dc.contributor.authorJones, J
dc.contributor.authorKaye, AH
dc.contributor.authorMorokoff, AP
dc.contributor.authorLuwor, RB
dc.date.accessioned2020-11-17T04:09:29Z
dc.date.available2020-11-17T04:09:29Z
dc.date.issued2020-10-20
dc.identifierpii: 10.1038/s41598-020-74746-x
dc.identifier.citationAreeb, Z., Stuart, S. F., West, A. J., Gomez, J., Nguyen, H. P. T., Paradiso, L., Zulkifli, A., Jones, J., Kaye, A. H., Morokoff, A. P. & Luwor, R. B. (2020). Reduced EGFR and increased miR-221 is associated with increased resistance to temozolomide and radiotherapy in glioblastoma. SCIENTIFIC REPORTS, 10 (1), https://doi.org/10.1038/s41598-020-74746-x.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/251718
dc.description.abstractDespite aggressive treatment with temozolomide and radiotherapy and extensive research into alternative therapies there has been little improvement in Glioblastoma patient survival. Median survival time remains between 12 and 15 months mainly due to treatment resistance and tumor recurrence. In this study, we aimed to explore the underlying mechanisms behind treatment resistance and the lack of success with anti-EGFR therapy in the clinic. After generating a number of treatment resistant Glioblastoma cell lines we observed that resistant cell lines lacked EGFR activation and expression. Furthermore, cell viability assays showed resistant cells were significantly less sensitive to the anti-EGFR agents when compared to parental cell lines. To further characterise the resistance mechanism in our cells microRNA prediction software identified miR-221 as a negative regulator of EGFR expression. miR-221 was up-regulated in our resistant cell lines, and this up-regulation led to a significant reduction in EGFR expression in both our cultured cell lines and a large cohort of glioblastoma patient tumor tissue.
dc.languageEnglish
dc.publisherNATURE RESEARCH
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleReduced EGFR and increased miR-221 is associated with increased resistance to temozolomide and radiotherapy in glioblastoma
dc.typeJournal Article
dc.identifier.doi10.1038/s41598-020-74746-x
melbourne.affiliation.departmentSurgery (RMH)
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.department
melbourne.source.titleScientific Reports
melbourne.source.volume10
melbourne.source.issue1
melbourne.source.pages17768-
dc.rights.licenseCC BY
melbourne.elementsid1469243
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576591
melbourne.contributor.authorMorokoff, Andrew
melbourne.contributor.authorLuwor, Rodney
melbourne.contributor.authorNguyen, Hong
melbourne.contributor.authorParadiso, Lucia
melbourne.contributor.authorJones, Jordan
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


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