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dc.contributor.authorGantner, CW
dc.contributor.authorCota-Coronado, A
dc.contributor.authorThompson, LH
dc.contributor.authorParish, CL
dc.date.accessioned2020-11-17T04:14:11Z
dc.date.available2020-11-17T04:14:11Z
dc.date.issued2020-09-18
dc.identifierpii: S2666-1667(20)30052-6
dc.identifier.citationGantner, C. W., Cota-Coronado, A., Thompson, L. H. & Parish, C. L. (2020). An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions.. STAR Protoc, 1 (2), pp.100065-. https://doi.org/10.1016/j.xpro.2020.100065.
dc.identifier.issn2666-1667
dc.identifier.urihttp://hdl.handle.net/11343/251743
dc.description.abstractHere, we describe a xeno-free, feeder-free, and chemically defined protocol for the generation of ventral midbrain dopaminergic (vmDA) progenitors from human pluripotent stem cells (hPSCs). This simple-to-follow protocol results in high yields of cryopreservable dopamine neurons across multiple hPSC lines. Wnt signaling is the critical component of the differentiation and can be finely adjusted in a line-dependent manner to enhance production of dopamine neurons for the purposes of transplantation, studying development and homeostasis, disease modeling, drug discovery, and drug development. For complete details on the use and execution of this protocol, please refer to Gantner et al. (2020) and Niclis et al. (2017a).
dc.languageeng
dc.publisherElsevier BV
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.titleAn Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions.
dc.typeJournal Article
dc.identifier.doi10.1016/j.xpro.2020.100065
melbourne.affiliation.departmentAnatomy and Neuroscience
melbourne.source.titleSTAR Protocols
melbourne.source.volume1
melbourne.source.issue2
melbourne.source.pages100065-
melbourne.identifier.nhmrc1102704
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1470628
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580226
melbourne.contributor.authorParish, Clare
melbourne.contributor.authorThompson, Lachlan
dc.identifier.eissn2666-1667
melbourne.identifier.fundernameidNHMRC, 1102704
melbourne.accessrightsOpen Access


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