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dc.contributor.authorChiri, E
dc.contributor.authorGreening, C
dc.contributor.authorLappan, R
dc.contributor.authorWaite, DW
dc.contributor.authorJirapanjawat, T
dc.contributor.authorDong, X
dc.contributor.authorArndt, SK
dc.contributor.authorNauer, PA
dc.date.accessioned2020-11-26T23:10:34Z
dc.date.available2020-11-26T23:10:34Z
dc.date.issued2020-07-24
dc.identifierpii: 10.1038/s41396-020-0722-3
dc.identifier.citationChiri, E., Greening, C., Lappan, R., Waite, D. W., Jirapanjawat, T., Dong, X., Arndt, S. K. & Nauer, P. A. (2020). Termite mounds contain soil-derived methanotroph communities kinetically adapted to elevated methane concentrations. The ISME Journal: multidisciplinary journal of microbial ecology, 14 (11), pp.2715-2731. https://doi.org/10.1038/s41396-020-0722-3.
dc.identifier.issn1751-7362
dc.identifier.urihttp://hdl.handle.net/11343/252135
dc.description.abstractTermite mounds have recently been confirmed to mitigate approximately half of termite methane (CH4) emissions, but the aerobic CH4 oxidising bacteria (methanotrophs) responsible for this consumption have not been resolved. Here, we describe the abundance, composition and CH4 oxidation kinetics of the methanotroph communities in the mounds of three distinct termite species sampled from Northern Australia. Results from three independent methods employed show that methanotrophs are rare members of microbial communities in termite mounds, with a comparable abundance but distinct composition to those of adjoining soil samples. Across all mounds, the most abundant and prevalent methane monooxygenase sequences were affiliated with upland soil cluster α (USCα), with sequences homologous to Methylocystis and tropical upland soil cluster (TUSC) also detected. The reconstruction of a metagenome-assembled genome of a mound USCα representative highlighted the metabolic capabilities of this group of methanotrophs. The apparent Michaelis–Menten kinetics of CH4 oxidation in mounds were estimated from in situ reaction rates. Methane affinities of the communities were in the low micromolar range, which is one to two orders of magnitude higher than those of upland soils, but significantly lower than those measured in soils with a large CH4 source such as landfill cover soils. The rate constant of CH4 oxidation, as well as the porosity of the mound material, were significantly positively correlated with the abundance of methanotroph communities of termite mounds. We conclude that termite-derived CH4 emissions have selected for distinct methanotroph communities that are kinetically adapted to elevated CH4 concentrations. However, factors other than substrate concentration appear to limit methanotroph abundance and hence these bacteria only partially mitigate termite-derived CH4 emissions. Our results also highlight the predominant role of USCα in an environment with elevated CH4 concentrations and suggest a higher functional diversity within this group than previously recognised.
dc.languageEnglish
dc.publisherSpringer Nature [academic journals on nature.com]
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleTermite mounds contain soil-derived methanotroph communities kinetically adapted to elevated methane concentrations
dc.typeJournal Article
dc.identifier.doi10.1038/s41396-020-0722-3
melbourne.affiliation.departmentSchool of Ecosystem and Forest Sciences
melbourne.source.titleThe ISME Journal: multidisciplinary journal of microbial ecology
melbourne.source.volume14
melbourne.source.issue11
melbourne.source.pages2715-2731
melbourne.identifier.arcDP120101735
dc.rights.licenseCC BY
melbourne.elementsid1459053
melbourne.contributor.authorNauer, Philipp
melbourne.contributor.authorArndt, Stefan
dc.identifier.eissn1751-7370
melbourne.identifier.fundernameidAustralian Research Council, DP120101735
melbourne.accessrightsOpen Access


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