Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow

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Hu, J; Zhang, W; Chauhan, SS; Shi, C; Song, Y; Zhao, Y; Wang, Z; Cheng, L; Zhang, YDate
2020-11-01Source Title
Journal of Veterinary SciencePublisher
KOREAN SOC VETERINARY SCIENCEAffiliation
Agriculture and Food SystemsMetadata
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Hu, J., Zhang, W., Chauhan, S. S., Shi, C., Song, Y., Zhao, Y., Wang, Z., Cheng, L. & Zhang, Y. (2020). Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow. JOURNAL OF VETERINARY SCIENCE, 21 (6), https://doi.org/10.4142/jvs.2020.21.e73.Access Status
Open AccessOpen Access at PMC
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710463Abstract
BACKGROUND: Bovine papilloma is a neoplastic disease caused by bovine papillomaviruses (BPVs), which were recently divided into 5 genera and at least 24 genotypes. OBJECTIVES: The complete genome sequence of BPV type 15 (BPV Aks-02), a novel putative BPV type from skin samples from infected cows in Southern Xinjiang China, was determined by collecting warty lesions, followed by DNA extraction and amplicon sequencing. METHODS: DNA was analyzed initially by polymerase chain reaction (PCR) using the degenerate primers FAP59 and FAP64. The complete genome sequences of the BPV Aks-02 were amplified by PCR using the amplification primers and sequencing primers. Sequence analysis and phylogenetic analysis were performed using bio-informatic software. RESULTS: The nucleotide sequence of the L1 open reading frame (ORF) of BPV Aks-02 was 75% identity to the L1 ORF of BPV-9 reference strain from GenBank. The complete genome consisted of 7,189 base pairs (G + C content of 42.50%) that encoded 5 early (E8, E7, E1, E2, and E4) and 2 late (L1 and L2) genes. The E7 protein contained a consensus CX₂CX29CX₂C zinc-binding domain and a LxCxE motif. Among the different members of this group, the percentages of the complete genome and ORFs (including 5 early and 2 late ORFs) sequence identity of BPV Aks-02 were closer to the genus Xipapillomavirus 1 of the Xipapillomavirus genus. Phylogenetic analysis and sequence similarities based on the L1 ORF of BPV Aks-02 revealed the same cluster. CONCLUSIONS: The results suggest that BPV type (BPV Aks-02) clustered with members of the Xipapillomavirus genus as BPV 15 and were closely related to Xipapillomavirus 1.
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