University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Veterinary and Agricultural Sciences
  • Agriculture and Food Systems
  • Agriculture and Food Systems - Research Publications
  • View Item
  • Minerva Access
  • Veterinary and Agricultural Sciences
  • Agriculture and Food Systems
  • Agriculture and Food Systems - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow

    Thumbnail
    Download
    Published version (2.064Mb)

    Citations
    Altmetric
    Author
    Hu, J; Zhang, W; Chauhan, SS; Shi, C; Song, Y; Zhao, Y; Wang, Z; Cheng, L; Zhang, Y
    Date
    2020-11-01
    Source Title
    Journal of Veterinary Science
    Publisher
    KOREAN SOC VETERINARY SCIENCE
    University of Melbourne Author/s
    Chauhan, Surinder Singh; Cheng, Long
    Affiliation
    Agriculture and Food Systems
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Hu, J., Zhang, W., Chauhan, S. S., Shi, C., Song, Y., Zhao, Y., Wang, Z., Cheng, L. & Zhang, Y. (2020). Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow. JOURNAL OF VETERINARY SCIENCE, 21 (6), https://doi.org/10.4142/jvs.2020.21.e73.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/252185
    DOI
    10.4142/jvs.2020.21.e73
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710463
    Abstract
    BACKGROUND: Bovine papilloma is a neoplastic disease caused by bovine papillomaviruses (BPVs), which were recently divided into 5 genera and at least 24 genotypes. OBJECTIVES: The complete genome sequence of BPV type 15 (BPV Aks-02), a novel putative BPV type from skin samples from infected cows in Southern Xinjiang China, was determined by collecting warty lesions, followed by DNA extraction and amplicon sequencing. METHODS: DNA was analyzed initially by polymerase chain reaction (PCR) using the degenerate primers FAP59 and FAP64. The complete genome sequences of the BPV Aks-02 were amplified by PCR using the amplification primers and sequencing primers. Sequence analysis and phylogenetic analysis were performed using bio-informatic software. RESULTS: The nucleotide sequence of the L1 open reading frame (ORF) of BPV Aks-02 was 75% identity to the L1 ORF of BPV-9 reference strain from GenBank. The complete genome consisted of 7,189 base pairs (G + C content of 42.50%) that encoded 5 early (E8, E7, E1, E2, and E4) and 2 late (L1 and L2) genes. The E7 protein contained a consensus CX₂CX29CX₂C zinc-binding domain and a LxCxE motif. Among the different members of this group, the percentages of the complete genome and ORFs (including 5 early and 2 late ORFs) sequence identity of BPV Aks-02 were closer to the genus Xipapillomavirus 1 of the Xipapillomavirus genus. Phylogenetic analysis and sequence similarities based on the L1 ORF of BPV Aks-02 revealed the same cluster. CONCLUSIONS: The results suggest that BPV type (BPV Aks-02) clustered with members of the Xipapillomavirus genus as BPV 15 and were closely related to Xipapillomavirus 1.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [45689]
    • Agriculture and Food Systems - Research Publications [542]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors