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dc.contributor.authorBruveris, FF
dc.contributor.authorNg, ES
dc.contributor.authorLeitoguinho, AR
dc.contributor.authorMotazedian, A
dc.contributor.authorVlahos, K
dc.contributor.authorSourris, K
dc.contributor.authorMayberry, R
dc.contributor.authorMcDonald, P
dc.contributor.authorAzzola, L
dc.contributor.authorDavidson, NM
dc.contributor.authorOshlack, A
dc.contributor.authorStanley, EG
dc.contributor.authorElefanty, AG
dc.date.accessioned2020-11-26T23:24:28Z
dc.date.available2020-11-26T23:24:28Z
dc.date.issued2020-10-01
dc.identifierpii: dev.193037
dc.identifier.citationBruveris, F. F., Ng, E. S., Leitoguinho, A. R., Motazedian, A., Vlahos, K., Sourris, K., Mayberry, R., McDonald, P., Azzola, L., Davidson, N. M., Oshlack, A., Stanley, E. G. & Elefanty, A. G. (2020). Human yolk sac-like haematopoiesis generates RUNX1-, GFI1- and/or GFI1B-dependent blood and SOX17-positive endothelium. Development, 147 (20), https://doi.org/10.1242/dev.193037.
dc.identifier.issn0950-1991
dc.identifier.urihttp://hdl.handle.net/11343/252215
dc.description.abstractThe genetic regulatory network controlling early fate choices during human blood cell development are not well understood. We used human pluripotent stem cell reporter lines to track the development of endothelial and haematopoietic populations in an in vitro model of human yolk-sac development. We identified SOX17−CD34+CD43− endothelial cells at day 2 of blast colony development, as a haemangioblast-like branch point from which SOX17−CD34+CD43+ blood cells and SOX17+CD34+CD43− endothelium subsequently arose. Most human blood cell development was dependent on RUNX1. Deletion of RUNX1 only permitted a single wave of yolk sac-like primitive erythropoiesis, but no yolk sac myelopoiesis or aorta-gonad-mesonephros (AGM)-like haematopoiesis. Blocking GFI1 and/or GFI1B activity with a small molecule inhibitor abrogated all blood cell development, even in cell lines with an intact RUNX1 gene. Together, our data define the hierarchical requirements for RUNX1, GFI1 and/or GFI1B during early human haematopoiesis arising from a yolk sac-like SOX17-negative haemogenic endothelial intermediate.
dc.languageEnglish
dc.publisherCompany Biologists Ltd
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleHuman yolk sac-like haematopoiesis generates RUNX1-, GFI1- and/or GFI1B-dependent blood and SOX17-positive endothelium
dc.typeJournal Article
dc.identifier.doi10.1242/dev.193037
melbourne.affiliation.departmentSchool of BioSciences
melbourne.affiliation.departmentSchool of Physics
melbourne.affiliation.departmentPaediatrics (RCH)
melbourne.source.titleDevelopment (Cambridge)
melbourne.source.volume147
melbourne.source.issue20
melbourne.source.pagesdev193037-dev193037
dc.rights.licenseCC BY
melbourne.elementsid1467907
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648599
melbourne.contributor.authorDavidson, Nadia
melbourne.contributor.authorOshlack, Alicia
melbourne.contributor.authorElefanty, Andrew
melbourne.contributor.authorStanley, Edouard
melbourne.contributor.authorBruveris, Freya
melbourne.contributor.authorLeitoguinho, Ana Rita
melbourne.contributor.authorMotazedian, Ali
dc.identifier.eissn1477-9129
melbourne.accessrightsOpen Access


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