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    Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase

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    Author
    Hakami, NY; Dusting, GJ; Chan, EC; Shah, MH; Peshavariya, HM
    Date
    2020-10-01
    Source Title
    Investigative Ophthalmology and Visual Science
    Publisher
    ASSOC RESEARCH VISION OPHTHALMOLOGY INC
    University of Melbourne Author/s
    Chan, Elsa; Dusting, Gregory; Hakami, Nora
    Affiliation
    Surgery (St Vincent's)
    Ophthalmology (Eye & Ear Hospital)
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Hakami, N. Y., Dusting, G. J., Chan, E. C., Shah, M. H. & Peshavariya, H. M. (2020). Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 61 (12), https://doi.org/10.1167/iovs.61.12.20.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/252246
    DOI
    10.1167/iovs.61.12.20
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585390
    Abstract
    Purpose: Corneal injury that occurs after burning with alkali initiates wound-healing processes, including inflammation, neovascularization, and fibrosis. Excessive reactions to injury can reduce corneal transparency and thereby compromise vision. The NADPH oxidase (Nox) enzyme complex is known to be involved in cell signaling for wound-healing angiogenesis, but its role in corneal neovascularization has been little studied. Methods: The center corneas of wild-type and Nox4 knockout (KO) mice were injured with 3 µL 1 M NaOH, while the contralateral corneas remained untouched. On day 7, mRNA expression levels of NADPH oxidase isoforms, the proangiogenic factors VEGF-A and TGFβ1, and proinflammatory genes ICAM-1 and VCAM-1 were determined. Corneal neovascularization and fibrosis were visualized using PECAM-1 antibody and picrosirius red staining, respectively, on the same day. Results: Expressions of both Nox2 and Nox4 gene isoforms as well as the above genes were markedly increased in the injured corneas at 7 days. Injured corneas showed neovascularization and fibrosis as well as an increase in clinical opacity score. All responses stimulated by alkali burn were abrogated in Nox4 KO mice. Conclusions: Nox4 could be a new target to treat pathologic corneal wound-healing responses and such targeting might prevent blindness caused by burn injuries.

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