Insights into pneumococcal pneumonia using lung aspirates and nasopharyngeal swabs collected from pneumonia patients in The Gambia.

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    Author
    Dunne, EM; Hua, Y; Salaudeen, R; Hossain, I; Ndiaye, M; Ortika, BD; Mulholland, EK; Hinds, J; Manna, S; Mackenzie, GA; ...
    Date
    2020-04-22
    Source Title
    Journal of Infectious Diseases
    Publisher
    Oxford University Press (OUP)
    University of Melbourne Author/s
    Satzke, Catherine; Mulholland, Edward; Dunne, Eileen; Manna, Salvatore
    Affiliation
    Paediatrics (RCH)
    Metadata
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    Document Type
    Journal Article
    Citations
    Dunne, E. M., Hua, Y., Salaudeen, R., Hossain, I., Ndiaye, M., Ortika, B. D., Mulholland, E. K., Hinds, J., Manna, S., Mackenzie, G. A. & Satzke, C. (2020). Insights into pneumococcal pneumonia using lung aspirates and nasopharyngeal swabs collected from pneumonia patients in The Gambia.. J Infect Dis, https://doi.org/10.1093/infdis/jiaa186.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/252390
    DOI
    10.1093/infdis/jiaa186
    Abstract
    We investigated the pathogenesis of pneumococcal pneumonia using clinical specimens collected for pneumonia surveillance in The Gambia. Lung aspirates and nasopharyngeal swabs from 31 patients were examined by culture, qPCR, whole genome sequencing, serotyping, and reverse transcription qPCR. Five lung aspirates cultured pneumococci, with a matching strain identified in the nasopharynx. Three virulence genes including ply (pneumolysin) were upregulated >20-fold in the lung compared with the nasopharynx. Nasopharyngeal pneumococcal density was higher in pediatric pneumonia patients compared with controls (p <0.0001). Findings suggest that changes in pneumococcal gene expression occurring in the lung environment may be important in pathogenesis.

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