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dc.contributor.authorLing, L
dc.contributor.authorRen, X
dc.contributor.authorCao, X
dc.contributor.authorHassan, ABM
dc.contributor.authorMah, S
dc.contributor.authorSathiyanathan, P
dc.contributor.authorSmith, RAA
dc.contributor.authorTan, CLL
dc.contributor.authorEio, M
dc.contributor.authorSamsonraj, RM
dc.contributor.authorvan Wijnen, AJ
dc.contributor.authorRaghunath, M
dc.contributor.authorNurcombe, V
dc.contributor.authorHui, JH
dc.contributor.authorCool, SM
dc.date.accessioned2020-11-27T00:38:05Z
dc.date.available2020-11-27T00:38:05Z
dc.date.issued2020-01-14
dc.identifierpii: S2213-6711(19)30417-5
dc.identifier.citationLing, L., Ren, X., Cao, X., Hassan, A. B. M., Mah, S., Sathiyanathan, P., Smith, R. A. A., Tan, C. L. L., Eio, M., Samsonraj, R. M., van Wijnen, A. J., Raghunath, M., Nurcombe, V., Hui, J. H. & Cool, S. M. (2020). Enhancing the Efficacy of Stem Cell Therapy with Glycosaminoglycans.. Stem Cell Reports, 14 (1), pp.105-121. https://doi.org/10.1016/j.stemcr.2019.12.003.
dc.identifier.issn2213-6711
dc.identifier.urihttp://hdl.handle.net/11343/252532
dc.description.abstractHuman mesenchymal stem cell (hMSC) therapy offers significant potential for osteochondral regeneration. Such applications require their ex vivo expansion in media frequently supplemented with fibroblast growth factor 2 (FGF2). Particular heparan sulfate (HS) fractions stabilize FGF2-FGF receptor complexes. We show that an FGF2-binding HS variant (HS8) accelerates the expansion of freshly isolated bone marrow hMSCs without compromising their naivety. Importantly, the repair of osteochondral defects in both rats and pigs is improved after treatment with HS8-supplemented hMSCs (MSCHS8), when assessed histologically, biomechanically, or by MRI. Thus, supplementing hMSC culture media with an HS variant that targets endogenously produced FGF2 allows the elimination of exogenous growth factors that may adversely affect their therapeutic potency.
dc.languageeng
dc.publisherElsevier BV
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.titleEnhancing the Efficacy of Stem Cell Therapy with Glycosaminoglycans.
dc.typeJournal Article
dc.identifier.doi10.1016/j.stemcr.2019.12.003
melbourne.affiliation.departmentMedicine Dentistry & Health Sciences
melbourne.source.titleStem Cell Reports
melbourne.source.volume14
melbourne.source.issue1
melbourne.source.pages105-121
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1462657
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962655
melbourne.contributor.authorMah, Sophia Ying Yii
dc.identifier.eissn2213-6711
melbourne.accessrightsOpen Access


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