Surface area-to-volume ratio, not cellular viscoelasticity, is the major determinant of red blood cell traversal through small channels.
AuthorNamvar, A; Blanch, AJ; Dixon, MW; Carmo, OMS; Liu, B; Tiash, S; Looker, O; Andrew, D; Chan, L-J; Tham, W-H; ...
Source TitleCellular Microbiology
University of Melbourne Author/sRajagopal, Vijayaraghavan; Tham, Wai; Tilley, Leann; Dixon, Matthew; Lee, Vee Sin; Tham, Wai-Hong; Blanch, Adam; Namvar, Arman; Carmo, Olivia
AffiliationBiochemistry and Molecular Biology
Medical Biology (W.E.H.I.)
Anatomy and Neuroscience
Document TypeJournal Article
CitationsNamvar, A., Blanch, A. J., Dixon, M. W., Carmo, O. M. S., Liu, B., Tiash, S., Looker, O., Andrew, D., Chan, L. -J., Tham, W. -H., Lee, P. V. S., Rajagopal, V. & Tilley, L. (2020). Surface area-to-volume ratio, not cellular viscoelasticity, is the major determinant of red blood cell traversal through small channels.. Cellular Microbiology, 23 (1), https://doi.org/10.1111/cmi.13270.
Access StatusOpen Access
ARC Grant codeARC/FL150100106
The remarkable deformability of red blood cells (RBCs) depends on the viscoelasticity of the plasma membrane and cell contents and the surface area to volume (SA:V) ratio; however, it remains unclear which of these factors is the key determinant for passage through small capillaries. We used a microfluidic device to examine the traversal of normal, stiffened, swollen, parasitised and immature RBCs. We show that dramatic stiffening of RBCs had no measurable effect on their ability to traverse small channels. By contrast, a moderate decrease in the SA:V ratio had a marked effect on the equivalent cylinder diameter that is traversable by RBCs of similar cellular viscoelasticity. We developed a finite element model that provides a coherent rationale for the experimental observations, based on the nonlinear mechanical behaviour of the RBC membrane skeleton. We conclude that the SA:V ratio should be given more prominence in studies of RBC pathologies.
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