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dc.contributor.authorWu, X
dc.contributor.authorBacic, A
dc.contributor.authorJohnson, KL
dc.contributor.authorHumphries, J
dc.date.accessioned2020-12-09T22:31:21Z
dc.date.available2020-12-09T22:31:21Z
dc.date.issued2020-11-01
dc.identifierpii: cells9112478
dc.identifier.citationWu, X., Bacic, A., Johnson, K. L. & Humphries, J. (2020). The Role of Brachypodium distachyon Wall-Associated Kinases (WAKs) in Cell Expansion and Stress Responses. CELLS, 9 (11), https://doi.org/10.3390/cells9112478.
dc.identifier.issn2073-4409
dc.identifier.urihttp://hdl.handle.net/11343/252990
dc.description.abstractThe plant cell wall plays a critical role in signaling responses to environmental and developmental cues, acting as both the sensing interface and regulator of plant cell integrity. Wall-associated kinases (WAKs) are plant receptor-like kinases located at the wall-plasma membrane-cytoplasmic interface and implicated in cell wall integrity sensing. WAKs in Arabidopsis thaliana have been shown to bind pectins in different forms under various conditions, such as oligogalacturonides (OG)s in stress response, and native pectin during cell expansion. The mechanism(s) WAKs use for sensing in grasses, which contain relatively low amounts of pectin, remains unclear. WAK genes from the model monocot plant, Brachypodium distachyon were identified. Expression profiling during early seedling development and in response to sodium salicylate and salt treatment was undertaken to identify WAKs involved in cell expansion and response to external stimuli. The BdWAK2 gene displayed increased expression during cell expansion and stress response, in addition to playing a potential role in the hypersensitive response. In vitro binding assays with various forms of commercial polysaccharides (pectins, xylans, and mixed-linkage glucans) and wall-extracted fractions (pectic/hemicellulosic/cellulosic) from both Arabidopsis and Brachypodium leaf tissues provided new insights into the binding properties of BdWAK2 and other candidate BdWAKs in grasses. The BdWAKs displayed a specificity for the acidic pectins with similar binding characteristics to the AtWAKs.
dc.languageEnglish
dc.publisherMDPI
dc.titleThe Role of Brachypodium distachyon Wall-Associated Kinases (WAKs) in Cell Expansion and Stress Responses
dc.typeJournal Article
dc.identifier.doi10.3390/cells9112478
melbourne.affiliation.departmentSchool of BioSciences
melbourne.source.titleCells
melbourne.source.volume9
melbourne.source.issue11
melbourne.source.pages2478-2478
dc.rights.licenseCC BY
melbourne.elementsid1480026
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698158
melbourne.contributor.authorJohnson, Kim
dc.identifier.eissn2073-4409
melbourne.accessrightsOpen Access


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