University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Paediatrics (RCH)
  • Paediatrics (RCH) - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Paediatrics (RCH)
  • Paediatrics (RCH) - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Measurement of Human Papillomavirus-Specific Antibodies Using a Pseudovirion-Based ELISA Method

    Thumbnail
    Download
    Published version (1.340Mb)

    Citations
    Altmetric
    Author
    Toh, ZQ; He, L; Chen, C; Huang, A; Russell, FM; Garland, SM; Reyburn, R; Ratu, T; Tuivaga, E; Frazer, IH; ...
    Date
    2020-10-28
    Source Title
    Frontiers in Immunology
    Publisher
    FRONTIERS MEDIA SA
    University of Melbourne Author/s
    Wark, Suzanne; Russell, Fiona; Mulholland, Edward; Toh, Zheng; Licciardi, Paul
    Affiliation
    Paediatrics (RCH)
    Obstetrics and Gynaecology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Toh, Z. Q., He, L., Chen, C., Huang, A., Russell, F. M., Garland, S. M., Reyburn, R., Ratu, T., Tuivaga, E., Frazer, I. H., Mulholland, E. K. & Licciardi, P. V. (2020). Measurement of Human Papillomavirus-Specific Antibodies Using a Pseudovirion-Based ELISA Method. FRONTIERS IN IMMUNOLOGY, 11, https://doi.org/10.3389/fimmu.2020.585768.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/252997
    DOI
    10.3389/fimmu.2020.585768
    Abstract
    Human papillomavirus (HPV) vaccines are safe and effective in preventing HPV infection and cervical precancers. Neutralizing antibodies are thought to be the primary mechanism of protection for HPV vaccines, although the exact level required for protection has not been identified. Three common serological assays used in clinical trials to measure HPV antibodies are HPV pseudovirion-based neutralization assay (PBNA), competitive or total Luminex immunoassays (cLIA or LIA) and VLP-based enzyme linked immunosorbent assays (ELISA). While PBNA is the gold-standard for measuring neutralizing antibodies (NAb), it is labor intensive. Luminex immunoassay and VLP-ELISA are rapid and high throughput, but their reagents and equipment can be difficult to source. Nevertheless, data generated from these assays generally correlate well with PBNA. Here, we described a simplified high-throughput PsV-based ELISA for HPV antibody measurement, to circumvent some of the limitations of existing assays. Using this assay, we were able to differentiate HPV-specific IgG and IgM, and found a strong correlation between HPV-specific IgG and NAb levels, as previously determined by PBNA. This assay platform is simpler and less time-consuming than PBNA. In addition, the materials can be readily produced and obtained commercially. This assay can be used as an alternative method to measure HPV antibodies.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [45770]
    • Obstetrics and Gynaecology - Research Publications [649]
    • Paediatrics (RCH) - Research Publications [1852]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors