Show simple item record

dc.contributor.authorTang, J
dc.contributor.authorChisholm, SA
dc.contributor.authorYeoh, LM
dc.contributor.authorGilson, PR
dc.contributor.authorPapenfuss, AT
dc.contributor.authorDay, KP
dc.contributor.authorPetter, M
dc.contributor.authorDuffy, MF
dc.date.accessioned2020-12-09T22:40:11Z
dc.date.available2020-12-09T22:40:11Z
dc.date.issued2020-12-23
dc.identifierpii: 10.1186/s13072-020-00365-5
dc.identifier.citationTang, J., Chisholm, S. A., Yeoh, L. M., Gilson, P. R., Papenfuss, A. T., Day, K. P., Petter, M. & Duffy, M. F. (2020). Histone modifications associated with gene expression and genome accessibility are dynamically enriched at Plasmodium falciparum regulatory sequences. EPIGENETICS & CHROMATIN, 13 (1), https://doi.org/10.1186/s13072-020-00365-5.
dc.identifier.issn1756-8935
dc.identifier.urihttp://hdl.handle.net/11343/253035
dc.description.abstractBACKGROUND: The malaria parasite Plasmodium falciparum has an unusually euchromatic genome with poorly conserved positioning of nucleosomes in intergenic sequences and poorly understood mechanisms of gene regulation. Variant histones and histone modifications determine nucleosome stability and recruit trans factors, but their combinatorial contribution to gene regulation is unclear. RESULTS: Here, we show that the histone H3 acetylations H3K18ac and H3K27ac and the variant histone Pf H2A.Z are enriched together at regulatory sites upstream of genes. H3K18ac and H3K27ac together dynamically mark regulatory regions of genes expressed during the asexual life cycle. In contrast, H3K4me1 is depleted in intergenic sequence and dynamically depleted upstream of expressed genes. The temporal pattern of H3K27ac and H3K18ac enrichment indicates that they accumulate during S phase and mitosis and are retained at regulatory sequences until at least G1 phase and after cessation of expression of the cognate genes. We integrated our ChIPseq data with existing datasets to show that in schizont stages H3K18ac, H3K27ac and Pf H2A.Z colocalise with the transcription factor PfAP2-I and the bromodomain protein PfBDP1 and are enriched at stably positioned nucleosomes within regions of exposed DNA at active transcriptional start sites. Using transient transfections we showed that sequences enriched with colocalised H3K18ac, H3K27ac and Pf H2A.Z possess promoter activity in schizont stages, but no enhancer-like activity. CONCLUSIONS: The dynamic H3 acetylations define P. falciparum regulatory sequences and contribute to gene activation. These findings expand the knowledge of the chromatin landscape that regulates gene expression in P. falciparum.
dc.languageEnglish
dc.publisherBMC
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleHistone modifications associated with gene expression and genome accessibility are dynamically enriched at Plasmodium falciparum regulatory sequences
dc.typeJournal Article
dc.identifier.doi10.1186/s13072-020-00365-5
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.departmentMedicine (RMH)
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleEpigenetics and Chromatin
melbourne.source.volume13
melbourne.source.issue1
melbourne.source.pages50-
melbourne.identifier.nhmrc1128975
melbourne.identifier.arcDP110100483
dc.rights.licenseCC BY
melbourne.elementsid1480728
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682024
melbourne.contributor.authorDuffy, Michael
melbourne.contributor.authorPetter, Michaela
melbourne.contributor.authorPapenfuss, Anthony
melbourne.contributor.authorDay, Karen
dc.identifier.eissn1756-8935
melbourne.identifier.fundernameidNHMRC, 1128975
melbourne.identifier.fundernameidAustralian Research Council, DP110100483
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record