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dc.contributor.authorLi, Y
dc.contributor.authorZhou, H
dc.contributor.authorCheng, L
dc.contributor.authorZhao, J
dc.contributor.authorHickford, J
dc.date.accessioned2020-12-09T22:40:42Z
dc.date.available2020-12-09T22:40:42Z
dc.date.issued2020-11-03
dc.identifierpii: 01021829
dc.identifier.citationLi, Y., Zhou, H., Cheng, L., Zhao, J. & Hickford, J. (2020). Variation in the stearoyl-CoA desaturase gene (SCD) and its influence on milk fatty acid composition in late-lactation dairy cattle grazed on pasture. Archives Animal Breeding, 63 (2), pp.355-366. https://doi.org/10.5194/aab-63-355-2020.
dc.identifier.issn0003-9438
dc.identifier.urihttp://hdl.handle.net/11343/253038
dc.description.abstractGene markers have become useful tools for improving animal genetics and breeding since they improve the accuracy of selection for superior breeding stock. In this study, the stearoyl-CoA desaturase (Δ-9-desaturase) gene (SCD) was investigated in New Zealand pasture-grazed Holstein–Friesian × Jersey cows. Three nucleotide substitutions were identified in exon 5 of the gene (c.702A/G, c.762T/C and c.878C/T), and a single nucleotide substitution was identified in intron 5 (c.880+105A/G). The c.878C/T substitution would, if expressed, result in the amino acid substitution p.A293V. Four nucleotide substitutions (c.*1783A/G, c.*1883C/T, c.*1984G/A and c.*2066T/C/G) were identified in the 3′-untranslated region (3′-UTR), and these resulted in three nucleotide sequence variants (named a, b and c). The sequence that would encode valine (V) at position 293 of SCD was linked to 3′-UTR variant a, and the sequence that would encode alanine (A) was linked to variants b and c. The frequency of the genotypes was as follows: VV (equivalent to aa: 15.1 %), VA (equivalent to ab+ac: 50.0 %) and AA (equivalent to bb+cc+bc: 34.9 %). The cows with the V variant produced less C10:1, C12:1 and C14:1 fatty acid (FA) but more C10:0, C11:0, C14:0, C16:1 and C18:2 FA than the A variant cows (P<0.001). Effects of c.*1783A/G and c.*2066T/C/G on milk fat composition were also found for the AA cows. The presence of c was associated with decreased levels of C16:1 (P<0.001), C17:1 (P=0.001), C18:2 cis-9, trans-13 (P=0.045), C18:2 cis-9, trans-12 (P=0.018) FA and C16:1 FA index (P<0.001). The presence of b was associated with increased levels of C13:0 iso FAs (P<0.001), monounsaturated FA (MUFA; P=0.002) and C12:1 (P<0.001).
dc.languageEnglish
dc.publisherCopernicus Publications
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleVariation in the stearoyl-CoA desaturase gene (SCD) and its influence on milk fatty acid composition in late-lactation dairy cattle grazed on pasture
dc.typeJournal Article
dc.identifier.doi10.5194/aab-63-355-2020
melbourne.affiliation.departmentAgriculture and Food Systems
melbourne.source.titleArchives Animal Breeding
melbourne.source.volume63
melbourne.source.issue2
melbourne.source.pages355-366
dc.rights.licenseCC BY
melbourne.elementsid1480931
melbourne.contributor.authorCheng, Long
dc.identifier.eissn2363-9822
melbourne.accessrightsOpen Access


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