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dc.contributor.authorKim, EJY
dc.contributor.authorAnko, M-L
dc.contributor.authorFlensberg, C
dc.contributor.authorMajewski, IJ
dc.contributor.authorGeng, F-S
dc.contributor.authorFiras, J
dc.contributor.authorHuang, DCS
dc.contributor.authorvan Delft, MF
dc.contributor.authorHeath, JK
dc.date.accessioned2020-12-09T22:57:56Z
dc.date.available2020-12-09T22:57:56Z
dc.date.issued2018-02-13
dc.identifierpii: S2213-6711(17)30567-2
dc.identifier.citationKim, E. J. Y., Anko, M. -L., Flensberg, C., Majewski, I. J., Geng, F. -S., Firas, J., Huang, D. C. S., van Delft, M. F. & Heath, J. K. (2018). BAK/BAX-Mediated Apoptosis Is a Myc-Induced Roadblock to Reprogramming. STEM CELL REPORTS, 10 (2), pp.331-338. https://doi.org/10.1016/j.stemcr.2017.12.019.
dc.identifier.issn2213-6711
dc.identifier.urihttp://hdl.handle.net/11343/253104
dc.description.abstractDespite intensive efforts to optimize the process, reprogramming differentiated cells to induced pluripotent stem cells (iPSCs) remains inefficient. The most common combination of transcription factors employed comprises OCT4, KLF4, SOX2, and MYC (OKSM). If MYC is omitted (OKS), reprogramming efficiency is reduced further. Cells must overcome several obstacles to reach the pluripotent state, one of which is apoptosis. To directly determine how extensively apoptosis limits reprogramming, we exploited mouse embryonic fibroblasts (MEFs) lacking the two essential mediators of apoptosis, BAK and BAX. Our results show that reprogramming is enhanced in MEFs deficient in BAK and BAX, but only when MYC is part of the reprogramming cocktail. Thus, the propensity for Myc overexpression to elicit apoptosis creates a significant roadblock to reprogramming under OKSM conditions. Our results suggest that blocking apoptosis during reprogramming may enhance the derivation of iPSCs for research and therapeutic purposes.
dc.languageEnglish
dc.publisherCELL PRESS
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.titleBAK/BAX-Mediated Apoptosis Is a Myc-Induced Roadblock to Reprogramming
dc.typeJournal Article
dc.identifier.doi10.1016/j.stemcr.2017.12.019
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.affiliation.departmentSurgery (RMH)
melbourne.source.titleStem Cell Reports
melbourne.source.volume10
melbourne.source.issue2
melbourne.source.pages331-338
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1300478
melbourne.contributor.authorvan Delft, Mark
melbourne.contributor.authorMajewski, Ian
melbourne.contributor.authorHuang, David
melbourne.contributor.authorHeath, Joan
melbourne.contributor.authorGeng, Fansuo
dc.identifier.eissn2213-6711
melbourne.accessrightsOpen Access


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