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dc.contributor.authorFakhri, O
dc.contributor.authorHartley, CA
dc.contributor.authorDevlin, JM
dc.contributor.authorBrowning, GF
dc.contributor.authorNoormohammadi, AH
dc.contributor.authorLee, S-W
dc.date.accessioned2020-12-09T23:02:51Z
dc.date.available2020-12-09T23:02:51Z
dc.date.issued2019-02-01
dc.identifierpii: 10.1007/s00705-018-4086-1
dc.identifier.citationFakhri, O., Hartley, C. A., Devlin, J. M., Browning, G. F., Noormohammadi, A. H. & Lee, S. -W. (2019). Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny. ARCHIVES OF VIROLOGY, 164 (2), pp.427-438. https://doi.org/10.1007/s00705-018-4086-1.
dc.identifier.issn0304-8608
dc.identifier.urihttp://hdl.handle.net/11343/253127
dc.description.abstractLive attenuated vaccines against infectious laryngotracheitis virus (ILTV) are widely used in the poultry industry to control disease and help prevent economic losses. Molecular epidemiological studies of currently circulating strains of ILTV within poultry flocks in Australia have demonstrated the presence of highly virulent viruses generated by genomic recombination events between vaccine strains. In this study, high-resolution melting (HRM) analysis was used to develop a tool to classify ILTV isolates and to investigate ILTV recombination. The assay was applied to plaque-purified progeny viruses generated after co-infection of chicken embryo kidney (CEK) monolayers with the A20 and Serva ILT vaccine strains and also to viruses isolated from field samples. The results showed that the HRM analysis is a suitable tool for the classification of ILTV isolates and can be used to detect recombination between ILTV vaccine strains in vitro. This method can be used to classify a broad range of ILTV strains to facilitate the classification and genotyping of ILTV and help to further understand recombination in these viruses.
dc.languageEnglish
dc.publisherSPRINGER WIEN
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleDevelopment and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny
dc.typeJournal Article
dc.identifier.doi10.1007/s00705-018-4086-1
melbourne.affiliation.departmentVeterinary Biosciences
melbourne.source.titleArchives of Virology
melbourne.source.volume164
melbourne.source.issue2
melbourne.source.pages427-438
melbourne.identifier.arcDE140100735
dc.rights.licenseCC BY
melbourne.elementsid1355931
pubs.publisher-urlhttps://www.ncbi.nlm.nih.gov/pubmed/30421085
melbourne.contributor.authorHadjinoormohammadi, Amir
melbourne.contributor.authorBrowning, Glenn
melbourne.contributor.authorLee, Sang-Won
melbourne.contributor.authorDevlin, Joanne
melbourne.contributor.authorHartley, Carol
melbourne.contributor.authorFakhri, Omid
melbourne.contributor.authorFakhri, Omid
dc.identifier.eissn1432-8798
melbourne.identifier.fundernameidAustralian Research Council, DE140100735
melbourne.accessrightsOpen Access


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