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dc.contributor.authorZhu, L
dc.contributor.authorShahid, MA
dc.contributor.authorMarkham, J
dc.contributor.authorBrowning, GF
dc.contributor.authorNoormohammadi, AH
dc.contributor.authorMarenda, MS
dc.date.accessioned2020-12-09T23:17:34Z
dc.date.available2020-12-09T23:17:34Z
dc.date.issued2018-02-02
dc.identifierpii: 10.1186/s12864-018-4501-8
dc.identifier.citationZhu, L., Shahid, M. A., Markham, J., Browning, G. F., Noormohammadi, A. H. & Marenda, M. S. (2018). Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution. BMC Genomics, 19 (1), https://doi.org/10.1186/s12864-018-4501-8.
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/11343/253184
dc.description.abstractBackground The bacterial pathogen Mycoplasma synoviae can cause subclinical respiratory disease, synovitis, airsacculitis and reproductive tract disease in poultry and is a major cause of economic loss worldwide. The M. synoviae strain MS-H was developed by chemical mutagenesis of an Australian isolate and has been used as a live attenuated vaccine in many countries over the past two decades. As a result it may now be the most prevalent strain of M. synoviae globally. Differentiation of the MS-H vaccine from local field strains is important for epidemiological investigations and is often required for registration of the vaccine. Results The complete genomic sequence of the MS-H strain was determined using a combination of Illumina and Nanopore methods and compared to WVU-1853, the M. synoviae type strain isolated in the USA 30 years before the parent strain of MS-H, and MS53, a more recent isolate from Brazil. The vaccine strain genome had a slightly larger number of pseudogenes than the two other strains and contained a unique 55 kb chromosomal inversion partially affecting a putative genomic island. Variations in gene content were also noted, including a deoxyribose-phosphate aldolase (deoC) fragment and an ATP-dependent DNA helicase gene found only in MS-H. Some of these sequences may have been acquired horizontally from other avian mycoplasma species. Conclusions MS-H was somewhat more similar to WVU-1853 than to MS53. The genome sequence of MS-H will enable identification of vaccine-specific genetic markers for use as diagnostic and epidemiological tools to better control M. synoviae.
dc.languageEnglish
dc.publisherBioMed Central
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleGenome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution
dc.typeJournal Article
dc.identifier.doi10.1186/s12864-018-4501-8
melbourne.affiliation.departmentVeterinary Biosciences
melbourne.affiliation.departmentElectrical and Electronic Engineering
melbourne.source.titleBMC Genomics
melbourne.source.volume19
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1305568
melbourne.contributor.authorMarenda, Marc
melbourne.contributor.authorZhu, Ling
melbourne.contributor.authorBrowning, Glenn
melbourne.contributor.authorHadjinoormohammadi, Amir
melbourne.contributor.authorMarkham, John
dc.identifier.eissn1471-2164
melbourne.accessrightsOpen Access


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