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dc.contributor.authorAndreadaki, M
dc.contributor.authorHanssen, E
dc.contributor.authorDeligianni, E
dc.contributor.authorClaudet, C
dc.contributor.authorWengelnik, K
dc.contributor.authorMollard, V
dc.contributor.authorMcFadden, GI
dc.contributor.authorAbkarian, M
dc.contributor.authorBraun-Breton, C
dc.contributor.authorSiden-Kiamos, I
dc.date.accessioned2020-12-09T23:22:52Z
dc.date.available2020-12-09T23:22:52Z
dc.date.issued2018-02-23
dc.identifierpii: 10.1038/s41598-018-21801-3
dc.identifier.citationAndreadaki, M., Hanssen, E., Deligianni, E., Claudet, C., Wengelnik, K., Mollard, V., McFadden, G. I., Abkarian, M., Braun-Breton, C. & Siden-Kiamos, I. (2018). Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell. SCIENTIFIC REPORTS, 8 (1), https://doi.org/10.1038/s41598-018-21801-3.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11343/253206
dc.description.abstractMalaria parasites alternate between intracellular and extracellular stages and successful egress from the host cell is crucial for continuation of the life cycle. We investigated egress of Plasmodium berghei gametocytes, an essential process taking place within a few minutes after uptake of a blood meal by the mosquito. Egress entails the rupture of two membranes surrounding the parasite: the parasitophorous vacuole membrane (PVM), and the red blood cell membrane (RBCM). High-speed video microscopy of 56 events revealed that egress in both genders comprises four well-defined phases, although each event is slightly different. The first phase is swelling of the host cell, followed by rupture and immediate vesiculation of the PVM. These vesicles are extruded through a single stabilized pore of the RBCM, and the latter is subsequently vesiculated releasing the free gametes. The time from PVM vesiculation to completion of egress varies between events. These observations were supported by immunofluorescence microscopy using antibodies against proteins of the RBCM and PVM. The combined results reveal dynamic re-organization of the membranes and the cortical cytoskeleton of the erythrocyte during egress.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleSequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell
dc.typeJournal Article
dc.identifier.doi10.1038/s41598-018-21801-3
melbourne.affiliation.departmentSchool of BioSciences
melbourne.affiliation.departmentBio21
melbourne.affiliation.departmentBiochemistry and Molecular Biology
melbourne.source.titleScientific Reports
melbourne.source.volume8
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1307181
melbourne.contributor.authorHanssen, Eric
melbourne.contributor.authorMollard, Vanessa
melbourne.contributor.authorMcFadden, Geoffrey
melbourne.contributor.authorSIDEN-KIAMOS, INGA
dc.identifier.eissn2045-2322
melbourne.accessrightsOpen Access


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