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    Single-particle imaging without symmetry constraints at an X-ray free-electron laser

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    Author
    Rose, M; Bobkov, S; Ayyer, K; Kurta, RP; Dzhigaev, D; Kim, YY; Morgan, AJ; Yoon, CH; Westphal, D; Bielecki, J; ...
    Date
    2018-11-01
    Source Title
    IUCrJ
    Publisher
    INT UNION CRYSTALLOGRAPHY
    University of Melbourne Author/s
    Morgan, Andrew
    Affiliation
    School of Physics
    Metadata
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    Document Type
    Journal Article
    Citations
    Rose, M., Bobkov, S., Ayyer, K., Kurta, R. P., Dzhigaev, D., Kim, Y. Y., Morgan, A. J., Yoon, C. H., Westphal, D., Bielecki, J., Sellberg, J. A., Williams, G., Maia, F. R. N. C., Yefanov, O. M., Ilyin, V., Mancuso, A. P., Chapman, H. N., Hogue, B. G., Aquila, A. ,... Vartanyants, I. A. (2018). Single-particle imaging without symmetry constraints at an X-ray free-electron laser. IUCRJ, 5 (Pt 6), pp.727-736. https://doi.org/10.1107/S205225251801120X.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/253354
    DOI
    10.1107/S205225251801120X
    Abstract
    The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.

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