Show simple item record

dc.contributor.authorMcCoy, PJ
dc.contributor.authorCostello, AJ
dc.contributor.authorCorcoran, NM
dc.contributor.authorHovens, CM
dc.contributor.authorClarkson, MJ
dc.date.accessioned2020-12-10T00:00:01Z
dc.date.available2020-12-10T00:00:01Z
dc.date.issued2019-01-01
dc.identifierpii: S2215-0161(18)30196-1
dc.identifier.citationMcCoy, P. J., Costello, A. J., Corcoran, N. M., Hovens, C. M. & Clarkson, M. J. (2019). Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation. METHODSX, 6, pp.22-33. https://doi.org/10.1016/j.mex.2018.11.015.
dc.identifier.issn2215-0161
dc.identifier.urihttp://hdl.handle.net/11343/253387
dc.description.abstractDNA-fluorescence in situ hybridisation (DNA-FISH) allows visualisation of chromosome organisation and rearrangement. FISH probes are pools of short fluorescently labelled DNA fragments that are often produced from template plasmids that contain large genomic inserts. For effective sample penetration and target hybridisation it is critical that probe fragments are between 200 and 500bp. Production of these short probes requires significant optimisation and can be confounded access to expensive sonication equipment or inherent sequence features that influence enzymatic fragmentation or amplification. Here we demonstrate that effective FISH probes can be prepared without the need for optimisation of fragmentation using a cocktail of two the 4bp recognition sequence restriction enzymes CviQI and AluI.
dc.languageEnglish
dc.publisherELSEVIER
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titlePreparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
dc.typeJournal Article
dc.identifier.doi10.1016/j.mex.2018.11.015
melbourne.affiliation.departmentSurgery (RMH)
melbourne.source.titleMethodsX
melbourne.source.volume6
melbourne.source.pages22-33
melbourne.identifier.nhmrc1047581
melbourne.identifier.nhmrc1104010
dc.rights.licenseCC BY
melbourne.elementsid1363355
melbourne.contributor.authorCorcoran, Niall
melbourne.contributor.authorCostello, Anthony
melbourne.contributor.authorHovens, Christopher
melbourne.contributor.authorClarkson, Michael
melbourne.contributor.authorMcCoy, Patrick
dc.identifier.eissn2215-0161
melbourne.identifier.fundernameidNHMRC, 1047581
melbourne.identifier.fundernameidNHMRC, 1104010
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record