Half-life-extended recombinant coagulation factor IX-albumin fusion protein is recycled via the FcRn-mediated pathway
AuthorChia, J; Louber, J; Glauser, I; Taylor, S; Bass, GT; Dower, SK; Gleeson, PA; Verhagen, AM
Source TitleJournal of Biological Chemistry
PublisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
University of Melbourne Author/sGleeson, Paul; Verhagen, Anne; Bass, Gregory; LOUBER, JADE; Dower, Steven
AffiliationBiochemistry and Molecular Biology
Document TypeJournal Article
CitationsChia, J., Louber, J., Glauser, I., Taylor, S., Bass, G. T., Dower, S. K., Gleeson, P. A. & Verhagen, A. M. (2018). Half-life-extended recombinant coagulation factor IX-albumin fusion protein is recycled via the FcRn-mediated pathway. JOURNAL OF BIOLOGICAL CHEMISTRY, 293 (17), pp.6363-6373. https://doi.org/10.1074/jbc.M117.817064.
Access StatusOpen Access
ARC Grant codeARC/LP130100531
The neonatal Fc receptor (FcRn) has a pivotal role in albumin and IgG homeostasis. Internalized IgG captured by FcRn under acidic endosomal conditions is recycled to the cell surface where exocytosis and a shift to neutral pH promote extracellular IgG release. Although a similar mechanism is proposed for FcRn-mediated albumin intracellular trafficking and recycling, this pathway is less well defined but is relevant to the development of therapeutics exploiting FcRn to extend the half-life of short-lived plasma proteins. Recently, a long-acting recombinant coagulation factor IX-albumin fusion protein (rIX-FP) has been approved for the management of hemophilia B. Fusion to albumin potentially enables internalized proteins to engage FcRn and escape lysosomal degradation. In this study, we present for the first time a detailed investigation of the FcRn-mediated recycling of albumin and the albumin fusion protein rIX-FP. We demonstrate that following internalization via FcRn at low pH, rIX-FP, like albumin, is detectable within the early endosome and rapidly (within 10-15 min) traffics into the Rab11+ recycling endosomes, from where it is exported from the cell. Similarly, rIX-FP and albumin taken up by fluid-phase endocytosis at physiological pH traffics into the Rab11+ recycling compartment in FcRn-positive cells but into the lysosomal compartment in FcRn-negative cells. As expected, recombinant factor IX (without albumin fusion) and an FcRn interaction-defective albumin variant localized to the lysosomal compartments of both FcRn-expressing and nonexpressing cells. These results indicate that FcRn-mediated recycling via the albumin moiety is a mechanism for the half-life extension of rIX-FP observed in clinical studies.
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