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dc.contributor.authorChen, Y
dc.contributor.authorKuroki, Y
dc.contributor.authorShaw, G
dc.contributor.authorPask, AJ
dc.contributor.authorYu, H
dc.contributor.authorToyoda, A
dc.contributor.authorFujiyama, A
dc.contributor.authorRenfree, MB
dc.date.accessioned2020-12-10T00:09:15Z
dc.date.available2020-12-10T00:09:15Z
dc.date.issued2019-03-01
dc.identifierpii: ncrna5010003
dc.identifier.citationChen, Y., Kuroki, Y., Shaw, G., Pask, A. J., Yu, H., Toyoda, A., Fujiyama, A. & Renfree, M. B. (2019). Androgen and Oestrogen Affect the Expression of Long Non-Coding RNAs During Phallus Development in a Marsupial. NON-CODING RNA, 5 (1), https://doi.org/10.3390/ncrna5010003.
dc.identifier.issn2311-553X
dc.identifier.urihttp://hdl.handle.net/11343/253426
dc.description.abstractThere is increasing evidence that long non-coding RNAs (lncRNAs) are important for normal reproductive development, yet very few lncRNAs have been identified in phalluses so far. Unlike eutherians, phallus development in the marsupial tammar wallaby occurs post-natally, enabling manipulation not possible in eutherians in which differentiation occurs in utero. We treated with sex steroids to determine the effects of androgen and oestrogen on lncRNA expression during phallus development. Hormonal manipulations altered the coding and non-coding gene expression profile of phalluses. We identified several predicted co-regulatory lncRNAs that appear to be co-expressed with the hormone-responsive candidate genes regulating urethral closure and phallus growth, namely IGF1, AR and ESR1. Interestingly, more than 50% of AR-associated coding genes and lncRNAs were also associated with ESR1. In addition, we identified and validated three novel co-regulatory and hormone-responsive lncRNAs: lnc-BMP5, lnc-ZBTB16 and lncRSPO4. Lnc-BMP5 was detected in the urethral epithelium of male phalluses and was downregulated by oestrogen in males. Lnc-ZBTB16 was downregulated by oestrogen treatment in male phalluses at day 50 post-partum (pp). LncRSPO4 was downregulated by adiol treatment in female phalluses but increased in male phalluses after castration. Thus, the expression pattern and hormone responsiveness of these lncRNAs suggests a physiological role in the development of the phallus.
dc.languageEnglish
dc.publisherMDPI
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleAndrogen and Oestrogen Affect the Expression of Long Non-Coding RNAs During Phallus Development in a Marsupial
dc.typeJournal Article
dc.identifier.doi10.3390/ncrna5010003
melbourne.affiliation.departmentSchool of BioSciences
melbourne.source.titleNon-Coding RNA
melbourne.source.volume5
melbourne.source.issue1
melbourne.identifier.nhmrc1138950
dc.rights.licenseCC BY
melbourne.elementsid1364165
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468475
melbourne.contributor.authorRenfree, Marilyn
melbourne.contributor.authorShaw, Geoffrey
melbourne.contributor.authorYu, Hongshi
melbourne.contributor.authorPask, Andrew
dc.identifier.eissn2311-553X
melbourne.identifier.fundernameidNHMRC, 1138950
melbourne.accessrightsOpen Access


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