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dc.contributor.authorJamaludin, SYN
dc.contributor.authorAzimi, I
dc.contributor.authorDavis, FM
dc.contributor.authorPeters, AA
dc.contributor.authorGonda, TJ
dc.contributor.authorThompson, EW
dc.contributor.authorRoberts-Thomson, SJ
dc.contributor.authorMonteith, GR
dc.date.accessioned2020-12-10T00:16:19Z
dc.date.available2020-12-10T00:16:19Z
dc.date.issued2018-04-01
dc.identifierpii: OL-0-0-7827
dc.identifier.citationJamaludin, S. Y. N., Azimi, I., Davis, F. M., Peters, A. A., Gonda, T. J., Thompson, E. W., Roberts-Thomson, S. J. & Monteith, G. R. (2018). Assessment of CXC ligand 12-mediated calcium signalling and its regulators in basal-like breast cancer cells. ONCOLOGY LETTERS, 15 (4), pp.4289-4295. https://doi.org/10.3892/ol.2018.7827.
dc.identifier.issn1792-1074
dc.identifier.urihttp://hdl.handle.net/11343/253447
dc.description.abstractCXC ligand (L)12 is a chemokine implicated in the migration, invasion and metastasis of cancer cells via interaction with its receptors CXC chemokine receptor (CXCR)4 and CXCR7. In the present study, CXCL12-mediated Ca2+ signalling was compared with two basal-like breast cancer cell lines, MDA-MB-231 and MDA-MB-468, which demonstrate distinct metastatic potential. CXCL12 treatment induced Ca2+ responses in the more metastatic MDA-MB-231 cells but not in the less metastatic MDA-MB-468 cells. Assessment of mRNA levels of CXCL12 receptors and their potential modulators in both cell lines revealed that CXCR4 and CXCR7 levels were increased in MDA-MB-231 cells compared with MDA-MB-468 cells. Cluster of differentiation (CD)24, the negative regulator of CXCL12 responses, demonstrated increased expression in MDA-MB-468 cells compared with MDA-MB-231 cells, and the two cell lines expressed comparable levels of hypoxia-inducible factor (HIF)2α, a CXCR4 regulator. Induction of epithelial-mesenchymal transition (EMT) by epidermal growth factor exhibited opposite effects on CXCR4 mRNA levels compared with hypoxia-induced EMT. Neither EMT inducer exhibited an effect on CXCR7 expression, however hypoxia increased HIF2α expression levels in MDA-MB-468 cells. Analysis of the gene expression profiles of breast tumours revealed that the highest expression levels of CXCR4 and CXCR7 were in the Claudin-Low molecular subtype, which is markedly associated with EMT features.
dc.languageEnglish
dc.publisherSPANDIDOS PUBL LTD
dc.titleAssessment of CXC ligand 12-mediated calcium signalling and its regulators in basal-like breast cancer cells
dc.typeJournal Article
dc.identifier.doi10.3892/ol.2018.7827
melbourne.affiliation.departmentSurgery (St Vincent's)
melbourne.source.titleOncology Letters
melbourne.source.volume15
melbourne.source.issue4
melbourne.source.pages4289-4295
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1316651
melbourne.contributor.authorThompson, Erik
dc.identifier.eissn1792-1082
melbourne.accessrightsOpen Access


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