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dc.contributor.authorCollins, DJ
dc.contributor.authorMorahan, B
dc.contributor.authorGarcia-Bustos, J
dc.contributor.authorDoerig, C
dc.contributor.authorPlebanski, M
dc.contributor.authorNeild, A
dc.date.accessioned2020-12-10T00:23:46Z
dc.date.available2020-12-10T00:23:46Z
dc.date.issued2015-11-01
dc.identifierpii: ncomms9686
dc.identifier.citationCollins, D. J., Morahan, B., Garcia-Bustos, J., Doerig, C., Plebanski, M. & Neild, A. (2015). Two-dimensional single-cell patterning with one cell per well driven by surface acoustic waves. NATURE COMMUNICATIONS, 6 (1), https://doi.org/10.1038/ncomms9686.
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/11343/253477
dc.description.abstractIn single-cell analysis, cellular activity and parameters are assayed on an individual, rather than population-average basis. Essential to observing the activity of these cells over time is the ability to trap, pattern and retain them, for which previous single-cell-patterning work has principally made use of mechanical methods. While successful as a long-term cell-patterning strategy, these devices remain essentially single use. Here we introduce a new method for the patterning of multiple spatially separated single particles and cells using high-frequency acoustic fields with one cell per acoustic well. We characterize and demonstrate patterning for both a range of particle sizes and the capture and patterning of cells, including human lymphocytes and red blood cells infected by the malarial parasite Plasmodium falciparum. This ability is made possible by a hitherto unexplored regime where the acoustic wavelength is on the same order as the cell dimensions.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleTwo-dimensional single-cell patterning with one cell per well driven by surface acoustic waves
dc.typeJournal Article
dc.identifier.doi10.1038/ncomms9686
melbourne.affiliation.departmentVeterinary Biosciences
melbourne.affiliation.departmentBiomedical Engineering
melbourne.source.titleNature Communications
melbourne.source.volume6
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1359247
melbourne.contributor.authorCollins, David
melbourne.contributor.authorGarcia-Bustos, Jose
dc.identifier.eissn2041-1723
melbourne.accessrightsOpen Access


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