A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells

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    Author
    Nefzger, CM; Jarde, T; Rossello, FJ; Horvay, K; Knaupp, AS; Powell, DR; Chen, J; Abud, HE; Polo, JM
    Date
    2016-03-08
    Source Title
    Stem Cell Reports
    Publisher
    CELL PRESS
    University of Melbourne Author/s
    Rossello, Fernando
    Affiliation
    Clinical Pathology
    Metadata
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    Document Type
    Journal Article
    Citations
    Nefzger, C. M., Jarde, T., Rossello, F. J., Horvay, K., Knaupp, A. S., Powell, D. R., Chen, J., Abud, H. E. & Polo, J. M. (2016). A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells. STEM CELL REPORTS, 6 (3), pp.321-329. https://doi.org/10.1016/j.stemcr.2016.01.014.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/253569
    DOI
    10.1016/j.stemcr.2016.01.014
    Abstract
    The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. Used on reporter-free mice, this strategy allows the isolation of functional, transcriptionally distinct ISCs uncompromised by Lgr5 haploinsufficiency.

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