A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells

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Author
Nefzger, CM; Jarde, T; Rossello, FJ; Horvay, K; Knaupp, AS; Powell, DR; Chen, J; Abud, HE; Polo, JMDate
2016-03-08Source Title
Stem Cell ReportsPublisher
CELL PRESSUniversity of Melbourne Author/s
Rossello, FernandoAffiliation
Clinical PathologyMetadata
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Journal ArticleCitations
Nefzger, C. M., Jarde, T., Rossello, F. J., Horvay, K., Knaupp, A. S., Powell, D. R., Chen, J., Abud, H. E. & Polo, J. M. (2016). A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells. STEM CELL REPORTS, 6 (3), pp.321-329. https://doi.org/10.1016/j.stemcr.2016.01.014.Access Status
Open AccessAbstract
The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. Used on reporter-free mice, this strategy allows the isolation of functional, transcriptionally distinct ISCs uncompromised by Lgr5 haploinsufficiency.
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