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dc.contributor.authorNefzger, CM
dc.contributor.authorJarde, T
dc.contributor.authorRossello, FJ
dc.contributor.authorHorvay, K
dc.contributor.authorKnaupp, AS
dc.contributor.authorPowell, DR
dc.contributor.authorChen, J
dc.contributor.authorAbud, HE
dc.contributor.authorPolo, JM
dc.date.accessioned2020-12-10T00:46:34Z
dc.date.available2020-12-10T00:46:34Z
dc.date.issued2016-03-08
dc.identifierpii: S2213-6711(16)00031-X
dc.identifier.citationNefzger, C. M., Jarde, T., Rossello, F. J., Horvay, K., Knaupp, A. S., Powell, D. R., Chen, J., Abud, H. E. & Polo, J. M. (2016). A Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells. STEM CELL REPORTS, 6 (3), pp.321-329. https://doi.org/10.1016/j.stemcr.2016.01.014.
dc.identifier.issn2213-6711
dc.identifier.urihttp://hdl.handle.net/11343/253569
dc.description.abstractThe isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. Used on reporter-free mice, this strategy allows the isolation of functional, transcriptionally distinct ISCs uncompromised by Lgr5 haploinsufficiency.
dc.languageEnglish
dc.publisherCELL PRESS
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.titleA Versatile Strategy for Isolating a Highly Enriched Population of Intestinal Stem Cells
dc.typeJournal Article
dc.identifier.doi10.1016/j.stemcr.2016.01.014
melbourne.affiliation.departmentClinical Pathology
melbourne.source.titleStem Cell Reports
melbourne.source.volume6
melbourne.source.issue3
melbourne.source.pages321-329
dc.rights.licenseCC BY-NC-ND
melbourne.elementsid1368191
melbourne.contributor.authorRossello, Fernando
melbourne.contributor.authorAbud, Helen
dc.identifier.eissn2213-6711
melbourne.accessrightsOpen Access


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