University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Veterinary and Agricultural Sciences
  • Melbourne Veterinary School
  • Veterinary Biosciences
  • Veterinary Biosciences - Research Publications
  • View Item
  • Minerva Access
  • Veterinary and Agricultural Sciences
  • Melbourne Veterinary School
  • Veterinary Biosciences
  • Veterinary Biosciences - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Whole genome sequence analysis of Australian avian pathogenic Escherichia coli that carry the class 1 integrase gene

    Thumbnail
    Download
    Published version (6.087Mb)

    Citations
    Scopus
    Web of Science
    Altmetric
    20
    16
    Author
    Cummins, ML; Reid, CJ; Chowdhury, PR; Bushell, RN; Esbert, N; Tivendale, KA; Noormohammadi, AH; Islam, S; Marenda, MS; Browning, GF; ...
    Date
    2019-02-01
    Source Title
    Microbial Genomics
    Publisher
    MICROBIOLOGY SOC
    University of Melbourne Author/s
    Browning, Glenn; Hadjinoormohammadi, Amir; Marenda, Marc; Tivendale, Kelly; Markham, Philip; Bushell, Rhys
    Affiliation
    Veterinary Biosciences
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Cummins, M. L., Reid, C. J., Chowdhury, P. R., Bushell, R. N., Esbert, N., Tivendale, K. A., Noormohammadi, A. H., Islam, S., Marenda, M. S., Browning, G. F., Markham, P. F. & Djordjevic, S. P. (2019). Whole genome sequence analysis of Australian avian pathogenic Escherichia coli that carry the class 1 integrase gene. MICROBIAL GENOMICS, 5 (2), https://doi.org/10.1099/mgen.0.000250.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/253571
    DOI
    10.1099/mgen.0.000250
    Abstract
    Avian pathogenic Escherichia coli (APEC) cause widespread economic losses in poultry production and are potential zoonotic pathogens. Genome sequences of 95 APEC from commercial poultry operations in four Australian states that carried the class 1 integrase gene intI1, a proxy for multiple drug resistance (MDR), were characterized. Sequence types ST117 (22/95), ST350 (10/95), ST429 and ST57 (each 9/95), ST95 (8/95) and ST973 (7/95) dominated, while 24 STs were represented by one or two strains. FII and FIB repA genes were the predominant (each 93/95, 98 %) plasmid incompatibility groups identified, but those of B/O/K/Z (25/95, 26 %) and I1 (24/95, 25 %) were also identified frequently. Virulence-associated genes (VAGs) carried by ColV and ColBM virulence plasmids, including those encoding protectins [iss (91/95, 96 %), ompT (91/95, 96 %) and traT (90/95, 95 %)], iron-acquisition systems [sitA (88/95, 93 %), etsA (87/95, 92 %), iroN (84/95, 89 %) and iucD/iutA (84/95, 89 %)] and the putative avian haemolysin hylF (91/95, 96 %), featured prominently. Notably, mobile resistance genes conferring resistance to fluoroquinolones, colistin, extended-spectrum β-lactams and carbapenems were not detected in the genomes of these 95 APEC but carriage of the sulphonamide resistance gene, sul1 (59/95, 63 %), the trimethoprim resistance gene cassettes dfrA5 (48/95, 50 %) and dfrA1 (25/95, 27 %), the tetracycline resistance determinant tet(A) (51/95, 55 %) and the ampicillin resistance genes blaTEM-1A/B/C (48/95, 52 %) was common. IS26 (77/95, 81 %), an insertion element known to capture and mobilize a wide spectrum of antimicrobial resistance genes, was also frequently identified. These studies provide a baseline snapshot of drug-resistant APEC in Australia and their role in the carriage of ColV-like virulence plasmids.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [45689]
    • Veterinary Biosciences - Research Publications [441]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors