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dc.contributor.authorBolden, JE
dc.contributor.authorLucas, EC
dc.contributor.authorZhou, G
dc.contributor.authorO'Sullivan, JA
dc.contributor.authorde Graaf, CA
dc.contributor.authorMcKenzie, MD
dc.contributor.authorDi Rago, L
dc.contributor.authorBaldwin, TM
dc.contributor.authorShortt, J
dc.contributor.authorAlexander, WS
dc.contributor.authorBochner, BS
dc.contributor.authorRitchie, ME
dc.contributor.authorHilton, DJ
dc.contributor.authorFairfax, KA
dc.date.accessioned2020-12-10T00:52:54Z
dc.date.available2020-12-10T00:52:54Z
dc.date.issued2018-07-01
dc.identifier.citationBolden, J. E., Lucas, E. C., Zhou, G., O'Sullivan, J. A., de Graaf, C. A., McKenzie, M. D., Di Rago, L., Baldwin, T. M., Shortt, J., Alexander, W. S., Bochner, B. S., Ritchie, M. E., Hilton, D. J. & Fairfax, K. A. (2018). Identification of a Siglec-F plus granulocyte-macrophage progenitor. JOURNAL OF LEUKOCYTE BIOLOGY, 104 (1), pp.123-133. https://doi.org/10.1002/JLB.1MA1217-475R.
dc.identifier.issn0741-5400
dc.identifier.urihttp://hdl.handle.net/11343/253598
dc.description.abstractIn recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils. Here, we describe a CD11b+ Siglec-F+ IL5Rα- myeloid population in the bone marrow of C57BL/6 mice. The CD11b+ Siglec-F+ IL5Rα- cells are retained in eosinophil deficient PHIL mice, and are not expanded upon overexpression of IL-5, indicating that they are upstream or independent of the eosinophil lineage. We show these cells to have GMP-like developmental potential in vitro and in vivo, and to be transcriptionally distinct from the classically described GMP population. The CD11b+ Siglec-F+ IL5Rα- population expands in the bone marrow of Myb mutant mice, which is potentially due to negative transcriptional regulation of Siglec-F by Myb. Lastly, we show that the role of Siglec-F may be, at least in part, to regulate GMP viability.
dc.languageEnglish
dc.publisherWILEY
dc.titleIdentification of a Siglec-F plus granulocyte-macrophage progenitor
dc.typeJournal Article
dc.identifier.doi10.1002/JLB.1MA1217-475R
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.affiliation.departmentClinical Pathology
melbourne.affiliation.departmentSir Peter MacCallum Department of Oncology
melbourne.source.titleJournal of Leukocyte Biology
melbourne.source.volume104
melbourne.source.issue1
melbourne.source.pages123-133
dc.rights.licenseCC BY
melbourne.elementsid1323250
melbourne.contributor.authorAlexander, Warren
melbourne.contributor.authorFairfax, Kirsten
melbourne.contributor.authorShortt, Jake
melbourne.contributor.authorHilton, Douglas
melbourne.contributor.authorBolden, Jessica
melbourne.contributor.authorde Graaf, Carolyn
melbourne.contributor.authorMcKenzie, Mark
melbourne.contributor.authorRitchie, Matthew
dc.identifier.eissn1938-3673
melbourne.accessrightsOpen Access


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