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    Mix-and-diffuse serial synchrotron crystallography

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    Author
    Beyerlein, KR; Dierksmeyer, D; Mariani, V; Kuhn, M; Sarrou, I; Ottaviano, A; Awel, S; Knoska, J; Fuglerud, S; Jonsson, O; ...
    Date
    2017-11-01
    Source Title
    IUCrJ
    Publisher
    INT UNION CRYSTALLOGRAPHY
    University of Melbourne Author/s
    Morgan, Andrew
    Affiliation
    School of Physics
    Metadata
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    Document Type
    Journal Article
    Citations
    Beyerlein, K. R., Dierksmeyer, D., Mariani, V., Kuhn, M., Sarrou, I., Ottaviano, A., Awel, S., Knoska, J., Fuglerud, S., Jonsson, O., Stern, S., Wiedorn, M. O., Yefanov, O., Adriano, L., Bean, R., Burkhardt, A., Fischer, P., Heymann, M., Horke, D. A. ,... Oberthuer, D. (2017). Mix-and-diffuse serial synchrotron crystallography. IUCRJ, 4 (Pt 6), pp.769-777. https://doi.org/10.1107/S2052252517013124.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/253728
    DOI
    10.1107/S2052252517013124
    Abstract
    Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. The success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.

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