Mix-and-diffuse serial synchrotron crystallography
AuthorBeyerlein, KR; Dierksmeyer, D; Mariani, V; Kuhn, M; Sarrou, I; Ottaviano, A; Awel, S; Knoska, J; Fuglerud, S; Jonsson, O; ...
PublisherINT UNION CRYSTALLOGRAPHY
University of Melbourne Author/sMorgan, Andrew
AffiliationSchool of Physics
Document TypeJournal Article
CitationsBeyerlein, K. R., Dierksmeyer, D., Mariani, V., Kuhn, M., Sarrou, I., Ottaviano, A., Awel, S., Knoska, J., Fuglerud, S., Jonsson, O., Stern, S., Wiedorn, M. O., Yefanov, O., Adriano, L., Bean, R., Burkhardt, A., Fischer, P., Heymann, M., Horke, D. A. ,... Oberthuer, D. (2017). Mix-and-diffuse serial synchrotron crystallography. IUCRJ, 4 (Pt 6), pp.769-777. https://doi.org/10.1107/S2052252517013124.
Access StatusOpen Access
Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. The success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.
- Click on "Export Reference in RIS Format" and choose "open with... Endnote".
- Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References