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dc.contributor.authorBeasley, JT
dc.contributor.authorBonneau, JP
dc.contributor.authorSanchez-Palacios, JT
dc.contributor.authorMoreno-Moyano, LT
dc.contributor.authorCallahan, DL
dc.contributor.authorTako, E
dc.contributor.authorGlahn, RP
dc.contributor.authorLombi, E
dc.contributor.authorJohnson, AAT
dc.date.accessioned2020-12-10T01:37:41Z
dc.date.available2020-12-10T01:37:41Z
dc.date.issued2019-08-01
dc.identifier.citationBeasley, J. T., Bonneau, J. P., Sanchez-Palacios, J. T., Moreno-Moyano, L. T., Callahan, D. L., Tako, E., Glahn, R. P., Lombi, E. & Johnson, A. A. T. (2019). Metabolic engineering of bread wheat improves grain iron concentration and bioavailability. PLANT BIOTECHNOLOGY JOURNAL, 17 (8), pp.1514-1526. https://doi.org/10.1111/pbi.13074.
dc.identifier.issn1467-7644
dc.identifier.urihttp://hdl.handle.net/11343/253747
dc.description.abstractBread wheat (Triticum aestivum L.) is cultivated on more land than any other crop and produces a fifth of the calories consumed by humans. Wheat endosperm is rich in starch yet contains low concentrations of dietary iron (Fe) and zinc (Zn). Biofortification is a micronutrient intervention aimed at increasing the density and bioavailability of essential vitamins and minerals in staple crops; Fe biofortification of wheat has proved challenging. In this study we employed constitutive expression (CE) of the rice (Oryza sativa L.) nicotianamine synthase 2 (OsNAS2) gene in bread wheat to up-regulate biosynthesis of two low molecular weight metal chelators - nicotianamine (NA) and 2'-deoxymugineic acid (DMA) - that play key roles in metal transport and nutrition. The CE-OsNAS2 plants accumulated higher concentrations of grain Fe, Zn, NA and DMA and synchrotron X-ray fluorescence microscopy (XFM) revealed enhanced localization of Fe and Zn in endosperm and crease tissues, respectively. Iron bioavailability was increased in white flour milled from field-grown CE-OsNAS2 grain and positively correlated with NA and DMA concentrations.
dc.languageEnglish
dc.publisherWILEY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleMetabolic engineering of bread wheat improves grain iron concentration and bioavailability
dc.typeJournal Article
dc.identifier.doi10.1111/pbi.13074
melbourne.affiliation.departmentSchool of BioSciences
melbourne.source.titlePlant Biotechnology Journal
melbourne.source.volume17
melbourne.source.issue8
melbourne.source.pages1514-1526
dc.rights.licenseCC BY
melbourne.elementsid1366539
melbourne.contributor.authorBonneau, Julien
melbourne.contributor.authorJohnson, Alexander
melbourne.contributor.authorBeasley, Jesse
melbourne.contributor.authorBeasley, Jesse
dc.identifier.eissn1467-7652
melbourne.accessrightsOpen Access


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