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    A Bioelectronic System to Measure the Glycolytic Metabolism of Activated CD4+ T Cells

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    Author
    Crowe, SM; Kintzios, S; Kaltsas, G; Palmer, CS
    Date
    2019-01-09
    Source Title
    Biosensors
    Publisher
    MDPI
    University of Melbourne Author/s
    Crowe, Suzanne; Palmer, Clovis
    Affiliation
    University General
    Microbiology and Immunology
    Metadata
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    Document Type
    Journal Article
    Citations
    Crowe, S. M., Kintzios, S., Kaltsas, G. & Palmer, C. S. (2019). A Bioelectronic System to Measure the Glycolytic Metabolism of Activated CD4+ T Cells. BIOSENSORS-BASEL, 9 (1), https://doi.org/10.3390/bios9010010.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/253754
    DOI
    10.3390/bios9010010
    Abstract
    The evaluation of glucose metabolic activity in immune cells is becoming an increasingly standard task in immunological research. In this study, we described a sensitive, inexpensive, and non-radioactive assay for the direct and rapid measurement of the metabolic activity of CD4+ T cells in culture. A portable, custom-built Cell Culture Metabolite Biosensor device was designed to measure the levels of acidification (a proxy for glycolysis) in cell-free CD4+ T cell culture media. In this assay, ex vivo activated CD4+ T cells were incubated in culture medium and mini electrodes were placed inside the cell free culture filtrates in 96-well plates. Using this technique, the inhibitors of glycolysis were shown to suppress acidification of the cell culture media, a response similar to that observed using a gold standard lactate assay kit. Our findings show that this innovative biosensor technology has potential for applications in metabolic research, where acquisition of sufficient cellular material for ex vivo analyses presents a substantial challenge.

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