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    Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics.

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    Author
    Yiangou, L; Grandy, RA; Morell, CM; Tomaz, RA; Osnato, A; Kadiwala, J; Muraro, D; Garcia-Bernardo, J; Nakanoh, S; Bernard, WG; ...
    Date
    2019-01-08
    Source Title
    Stem Cell Reports
    Publisher
    Elsevier BV
    University of Melbourne Author/s
    McCarthy, Davis
    Affiliation
    School of Mathematics and Statistics
    Metadata
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    Document Type
    Journal Article
    Citations
    Yiangou, L., Grandy, R. A., Morell, C. M., Tomaz, R. A., Osnato, A., Kadiwala, J., Muraro, D., Garcia-Bernardo, J., Nakanoh, S., Bernard, W. G., Ortmann, D., McCarthy, D. J., Simonic, I., Sinha, S. & Vallier, L. (2019). Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics.. Stem Cell Reports, 12 (1), pp.165-179. https://doi.org/10.1016/j.stemcr.2018.11.020.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/253766
    DOI
    10.1016/j.stemcr.2018.11.020
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335580
    Abstract
    Cell cycle progression and cell fate decisions are closely linked in human pluripotent stem cells (hPSCs). However, the study of these interplays at the molecular level remains challenging due to the lack of efficient methods allowing cell cycle synchronization of large quantities of cells. Here, we screened inhibitors of cell cycle progression and identified nocodazole as the most efficient small molecule to synchronize hPSCs in the G2/M phase. Following nocodazole treatment, hPSCs remain pluripotent, retain a normal karyotype and can successfully differentiate into the three germ layers and functional cell types. Moreover, genome-wide transcriptomic analyses on single cells synchronized for their cell cycle and differentiated toward the endoderm lineage validated our findings and showed that nocodazole treatment has no effect on gene expression during the differentiation process. Thus, our synchronization method provides a robust approach to study cell cycle mechanisms in hPSCs.

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