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dc.contributor.authorYiangou, L
dc.contributor.authorGrandy, RA
dc.contributor.authorMorell, CM
dc.contributor.authorTomaz, RA
dc.contributor.authorOsnato, A
dc.contributor.authorKadiwala, J
dc.contributor.authorMuraro, D
dc.contributor.authorGarcia-Bernardo, J
dc.contributor.authorNakanoh, S
dc.contributor.authorBernard, WG
dc.contributor.authorOrtmann, D
dc.contributor.authorMcCarthy, DJ
dc.contributor.authorSimonic, I
dc.contributor.authorSinha, S
dc.contributor.authorVallier, L
dc.date.accessioned2020-12-10T01:41:38Z
dc.date.available2020-12-10T01:41:38Z
dc.date.issued2019-01-08
dc.identifierpii: S2213-6711(18)30489-2
dc.identifier.citationYiangou, L., Grandy, R. A., Morell, C. M., Tomaz, R. A., Osnato, A., Kadiwala, J., Muraro, D., Garcia-Bernardo, J., Nakanoh, S., Bernard, W. G., Ortmann, D., McCarthy, D. J., Simonic, I., Sinha, S. & Vallier, L. (2019). Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics.. Stem Cell Reports, 12 (1), pp.165-179. https://doi.org/10.1016/j.stemcr.2018.11.020.
dc.identifier.issn2213-6711
dc.identifier.urihttp://hdl.handle.net/11343/253766
dc.description.abstractCell cycle progression and cell fate decisions are closely linked in human pluripotent stem cells (hPSCs). However, the study of these interplays at the molecular level remains challenging due to the lack of efficient methods allowing cell cycle synchronization of large quantities of cells. Here, we screened inhibitors of cell cycle progression and identified nocodazole as the most efficient small molecule to synchronize hPSCs in the G2/M phase. Following nocodazole treatment, hPSCs remain pluripotent, retain a normal karyotype and can successfully differentiate into the three germ layers and functional cell types. Moreover, genome-wide transcriptomic analyses on single cells synchronized for their cell cycle and differentiated toward the endoderm lineage validated our findings and showed that nocodazole treatment has no effect on gene expression during the differentiation process. Thus, our synchronization method provides a robust approach to study cell cycle mechanisms in hPSCs.
dc.languageeng
dc.publisherElsevier BV
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleMethod to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics.
dc.typeJournal Article
dc.identifier.doi10.1016/j.stemcr.2018.11.020
melbourne.affiliation.departmentSchool of Mathematics and Statistics
melbourne.source.titleStem Cell Reports
melbourne.source.volume12
melbourne.source.issue1
melbourne.source.pages165-179
dc.rights.licenseCC BY
melbourne.elementsid1370052
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335580
melbourne.contributor.authorMcCarthy, Davis
dc.identifier.eissn2213-6711
melbourne.accessrightsOpen Access


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