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    Intense interval training in healthy older adults increases skeletal muscle [H-3]ouabain-binding site content and elevates Na+,K+-ATPase alpha(2) isoform abundance in Type II fibers

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    Author
    Wyckelsma, VL; Levinger, I; Murphy, RM; Petersen, AC; Perry, BD; Hedges, CP; Anderson, MJ; McKenna, MJ
    Date
    2017-04-01
    Source Title
    Physiological Reports
    Publisher
    WILEY
    University of Melbourne Author/s
    Levinger, Itamar
    Affiliation
    Medicine and Radiology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Wyckelsma, V. L., Levinger, I., Murphy, R. M., Petersen, A. C., Perry, B. D., Hedges, C. P., Anderson, M. J. & McKenna, M. J. (2017). Intense interval training in healthy older adults increases skeletal muscle [H-3]ouabain-binding site content and elevates Na+,K+-ATPase alpha(2) isoform abundance in Type II fibers. PHYSIOLOGICAL REPORTS, 5 (7), https://doi.org/10.14814/phy2.13219.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/253798
    DOI
    10.14814/phy2.13219
    Abstract
    Young adults typically adapt to intense exercise training with an increased skeletal muscle Na+,K+-ATPase (NKA) content, concomitant with reduced extracellular potassium concentration [K+] during exercise and enhanced exercise performance. Whether these changes with longitudinal training occur in older adults is unknown and was investigated here. Fifteen older adults (69.4 ± 3.5 years, mean ± SD) were randomized to either 12 weeks of intense interval training (4 × 4 min at 90-95% peak heart rate), 3 days/week (IIT, n = 8); or no exercise controls (n = 7). Before and after training, participants completed an incremental cycle ergometer exercise test until a rating of perceived exertion of 17 (very hard) on a 20-point scale was attained, with measures of antecubital venous [K+]v Participants underwent a resting muscle biopsy prior to and at 48-72 h following the final training session. After IIT, the peak exercise work rate (25%), oxygen uptake (16%) and heart rate (6%) were increased (P < 0.05). After IIT, the peak exercise plasma [K+]v tended to rise (P = 0.07), while the rise in plasma [K+]v relative to work performed (nmol.L-1J-1) was unchanged. Muscle NKA content increased by 11% after IIT (P < 0.05). Single fiber measurements, increased in NKA α2 isoform in Type II fibers after IIT (30%, P < 0.05), with no changes to the other isoforms in single fibers or homogenate. Thus, intense exercise training in older adults induced an upregulation of muscle NKA, with a fiber-specific increase in NKA α2 abundance in Type II fibers, coincident with increased muscle NKA content and enhanced exercise performance.

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