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dc.contributor.authorDuarte, D
dc.contributor.authorAmarteifio, S
dc.contributor.authorAng, H
dc.contributor.authorKong, IY
dc.contributor.authorRuivo, N
dc.contributor.authorPruessner, G
dc.contributor.authorHawkins, ED
dc.contributor.authorLo Celso, C
dc.date.accessioned2020-12-10T01:50:59Z
dc.date.available2020-12-10T01:50:59Z
dc.date.issued2019-02-01
dc.identifier.citationDuarte, D., Amarteifio, S., Ang, H., Kong, I. Y., Ruivo, N., Pruessner, G., Hawkins, E. D. & Lo Celso, C. (2019). Defining the in vivo characteristics of acute myeloid leukemia cells behavior by intravital imaging. IMMUNOLOGY AND CELL BIOLOGY, 97 (2), pp.229-235. https://doi.org/10.1111/imcb.12216.
dc.identifier.issn0818-9641
dc.identifier.urihttp://hdl.handle.net/11343/253815
dc.description.abstractThe majority of acute myeloid leukemia (AML) patients have a poor response to conventional chemotherapy. The survival of chemoresistant cells is thought to depend on leukemia-bone marrow (BM) microenvironment interactions, which are not well understood. The CXCL12/CXCR4 axis has been proposed to support AML growth but was not studied at the single AML cell level. We recently showed that T-cell acute lymphoblastic leukemia (T-ALL) cells are highly motile in the BM; however, the characteristics of AML cell migration within the BM remain undefined. Here, we characterize the in vivo migratory behavior of AML cells and their response to chemotherapy and CXCR4 antagonism, using high-resolution 2-photon and confocal intravital microscopy of mouse calvarium BM and the well-established MLL-AF9-driven AML mouse model. We used the Notch1-driven T-ALL model as a benchmark comparison and AMD3100 for CXCR4 antagonism experiments. We show that AML cells are migratory, and in contrast with T-ALL, chemoresistant AML cells become less motile. Moreover, and in contrast with T-ALL, the in vivo exploratory behavior of expanding and chemoresistant AML cells is unaffected by AMD3100. These results expand our understanding of AML cells-BM microenvironment interactions, highlighting unique traits of leukemia of different lineages.
dc.languageEnglish
dc.publisherWILEY
dc.titleDefining the in vivo characteristics of acute myeloid leukemia cells behavior by intravital imaging
dc.typeJournal Article
dc.identifier.doi10.1111/imcb.12216
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.affiliation.department
melbourne.source.titleImmunology and Cell Biology
melbourne.source.volume97
melbourne.source.issue2
melbourne.source.pages229-235
dc.rights.licenseCC BY
melbourne.elementsid1357505
melbourne.contributor.authorHawkins, Edwin
melbourne.contributor.authorKong, Isabella Yingjia
dc.identifier.eissn1440-1711
melbourne.accessrightsOpen Access


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