A Liposomal Platform for Sensing of Extracellular Analytes Near Cells
AuthorZhang, X; Heng, S; Pei, J; Morey, JR; McDevitt, CA; Abell, AD
University of Melbourne Author/sMcDevitt, Christopher
AffiliationMicrobiology and Immunology
Document TypeJournal Article
CitationsZhang, X., Heng, S., Pei, J., Morey, J. R., McDevitt, C. A. & Abell, A. D. (2018). A Liposomal Platform for Sensing of Extracellular Analytes Near Cells. BIOSENSORS-BASEL, 8 (4), https://doi.org/10.3390/bios8040117.
Access StatusOpen Access
Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca2+, GSH, and Zn2+, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca2+ (0.25 mM⁻1 mM), Zn2+ (6.25 µM⁻50 µM), and GSH (0.25 mM⁻1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn2+ (1 mM), Ca2+ (1 mM), or GSH (1 mM) near cells without internalisation. This new sensing platform provides a means to repurpose a range of intracellular fluorescent sensors to specifically detect extracellular analytes, while also improving biocompatibility for overall enhanced use in a wide range of biomedical applications.
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