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dc.contributor.authorZhang, X
dc.contributor.authorHeng, S
dc.contributor.authorPei, J
dc.contributor.authorMorey, JR
dc.contributor.authorMcDevitt, CA
dc.contributor.authorAbell, AD
dc.date.accessioned2020-12-10T01:52:20Z
dc.date.available2020-12-10T01:52:20Z
dc.date.issued2018-12-01
dc.identifierpii: bios8040117
dc.identifier.citationZhang, X., Heng, S., Pei, J., Morey, J. R., McDevitt, C. A. & Abell, A. D. (2018). A Liposomal Platform for Sensing of Extracellular Analytes Near Cells. BIOSENSORS-BASEL, 8 (4), https://doi.org/10.3390/bios8040117.
dc.identifier.issn2079-6374
dc.identifier.urihttp://hdl.handle.net/11343/253823
dc.description.abstractCell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca2+, GSH, and Zn2+, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca2+ (0.25 mM⁻1 mM), Zn2+ (6.25 µM⁻50 µM), and GSH (0.25 mM⁻1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn2+ (1 mM), Ca2+ (1 mM), or GSH (1 mM) near cells without internalisation. This new sensing platform provides a means to repurpose a range of intracellular fluorescent sensors to specifically detect extracellular analytes, while also improving biocompatibility for overall enhanced use in a wide range of biomedical applications.
dc.languageEnglish
dc.publisherMDPI
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleA Liposomal Platform for Sensing of Extracellular Analytes Near Cells
dc.typeJournal Article
dc.identifier.doi10.3390/bios8040117
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titleBiosensors
melbourne.source.volume8
melbourne.source.issue4
melbourne.identifier.nhmrc1122582
melbourne.identifier.arcDP170102102
melbourne.identifier.arcFT170100006
dc.rights.licenseCC BY
melbourne.elementsid1358143
melbourne.contributor.authorMcDevitt, Christopher
dc.identifier.eissn2079-6374
melbourne.identifier.fundernameidNHMRC, 1122582
melbourne.identifier.fundernameidAustralian Research Council, DP170102102
melbourne.identifier.fundernameidAustralian Research Council, FT170100006
melbourne.accessrightsOpen Access


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