dc.contributor.author | Zhang, X | |
dc.contributor.author | Heng, S | |
dc.contributor.author | Pei, J | |
dc.contributor.author | Morey, JR | |
dc.contributor.author | McDevitt, CA | |
dc.contributor.author | Abell, AD | |
dc.date.accessioned | 2020-12-10T01:52:20Z | |
dc.date.available | 2020-12-10T01:52:20Z | |
dc.date.issued | 2018-12-01 | |
dc.identifier | pii: bios8040117 | |
dc.identifier.citation | Zhang, X., Heng, S., Pei, J., Morey, J. R., McDevitt, C. A. & Abell, A. D. (2018). A Liposomal Platform for Sensing of Extracellular Analytes Near Cells. BIOSENSORS-BASEL, 8 (4), https://doi.org/10.3390/bios8040117. | |
dc.identifier.issn | 2079-6374 | |
dc.identifier.uri | http://hdl.handle.net/11343/253823 | |
dc.description.abstract | Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca2+, GSH, and Zn2+, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca2+ (0.25 mM⁻1 mM), Zn2+ (6.25 µM⁻50 µM), and GSH (0.25 mM⁻1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn2+ (1 mM), Ca2+ (1 mM), or GSH (1 mM) near cells without internalisation. This new sensing platform provides a means to repurpose a range of intracellular fluorescent sensors to specifically detect extracellular analytes, while also improving biocompatibility for overall enhanced use in a wide range of biomedical applications. | |
dc.language | English | |
dc.publisher | MDPI | |
dc.title | A Liposomal Platform for Sensing of Extracellular Analytes Near Cells | |
dc.type | Journal Article | |
dc.identifier.doi | 10.3390/bios8040117 | |
melbourne.affiliation.department | Microbiology and Immunology | |
melbourne.source.title | Biosensors | |
melbourne.source.volume | 8 | |
melbourne.source.issue | 4 | |
dc.rights.license | CC BY | |
melbourne.elementsid | 1358143 | |
melbourne.contributor.author | McDevitt, Christopher | |
dc.identifier.eissn | 2079-6374 | |
melbourne.accessrights | Open Access | |