Automated Planar Patch-Clamp Recording of P2X Receptors.
AuthorMilligan, CJ; Jiang, L-H
Source TitleMethods in Molecular Biology
PublisherSpringer New York
University of Melbourne Author/sMilligan, Carol
AffiliationFlorey Department of Neuroscience and Mental Health
Document TypeJournal Article
CitationsMilligan, C. J. & Jiang, L. -H. (2020). Automated Planar Patch-Clamp Recording of P2X Receptors.. Methods Mol Biol, 2041, pp.285-300. https://doi.org/10.1007/978-1-4939-9717-6_21.
Access StatusAccess this item via the Open Access location
Open Access URLhttp://eprints.whiterose.ac.uk/154716/1/Automated%20planar%20patch-clamp%20recording%20of%20P2X%20receptors.pdf
P2X receptors are a structurally and functionally distinctive family of ligand-gated ion channels that play important roles in mediating extracellular adenosine 5'-triphosphate (ATP) signaling in diverse physiological and pathophysiological processes. For several decades, the "manual" patch-clamp technique was regarded as the gold standard assay for investigating ion channel properties. More recently, breakthroughs in the development of automated patch-clamp technologies are enabling the study of ion channels, with much greater throughput capacities. These automated platforms, of which there are many, generate consistent, reliable, high-fidelity data. This chapter demonstrates the versatility of one of these technologies for ligand-gated ion channels, with a particular emphasis on protocols that address some of the issues of receptor desensitization that are commonly associated with P2X receptor-mediated currents.
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