Automated Planar Patch-Clamp Recording of P2X Receptors.
Citations
Altmetric
Author
Milligan, CJ; Jiang, L-HDate
2020Source Title
Methods in Molecular BiologyPublisher
Springer New YorkUniversity of Melbourne Author/s
Milligan, CarolAffiliation
Florey Department of Neuroscience and Mental HealthMetadata
Show full item recordDocument Type
Journal ArticleCitations
Milligan, C. J. & Jiang, L. -H. (2020). Automated Planar Patch-Clamp Recording of P2X Receptors.. Methods Mol Biol, 2041, pp.285-300. https://doi.org/10.1007/978-1-4939-9717-6_21.Access Status
Access this item via the Open Access locationOpen Access URL
http://eprints.whiterose.ac.uk/154716/1/Automated%20planar%20patch-clamp%20recording%20of%20P2X%20receptors.pdfAbstract
P2X receptors are a structurally and functionally distinctive family of ligand-gated ion channels that play important roles in mediating extracellular adenosine 5'-triphosphate (ATP) signaling in diverse physiological and pathophysiological processes. For several decades, the "manual" patch-clamp technique was regarded as the gold standard assay for investigating ion channel properties. More recently, breakthroughs in the development of automated patch-clamp technologies are enabling the study of ion channels, with much greater throughput capacities. These automated platforms, of which there are many, generate consistent, reliable, high-fidelity data. This chapter demonstrates the versatility of one of these technologies for ligand-gated ion channels, with a particular emphasis on protocols that address some of the issues of receptor desensitization that are commonly associated with P2X receptor-mediated currents.
Export Reference in RIS Format
Endnote
- Click on "Export Reference in RIS Format" and choose "open with... Endnote".
Refworks
- Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References