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dc.contributor.authorSenders, ML
dc.contributor.authorMeerwaldt, AE
dc.contributor.authorvan Leent, MMT
dc.contributor.authorSanchez-Gaytan, BL
dc.contributor.authorvan de Voort, JC
dc.contributor.authorToner, YC
dc.contributor.authorMaier, A
dc.contributor.authorKlein, ED
dc.contributor.authorSullivan, NAT
dc.contributor.authorSofias, AM
dc.contributor.authorGroenen, H
dc.contributor.authorFaries, C
dc.contributor.authorOosterwijk, RS
dc.contributor.authorvan Leeuwen, EM
dc.contributor.authorFay, F
dc.contributor.authorChepurko, E
dc.contributor.authorReiner, T
dc.contributor.authorDuivenvoorden, R
dc.contributor.authorZangi, L
dc.contributor.authorDijkhuizen, RM
dc.contributor.authorHak, S
dc.contributor.authorSwirski, FK
dc.contributor.authorNahrendorf, M
dc.contributor.authorPérez-Medina, C
dc.contributor.authorTeunissen, AJP
dc.contributor.authorFayad, ZA
dc.contributor.authorCalcagno, C
dc.contributor.authorStrijkers, GJ
dc.contributor.authorMulder, WJM
dc.date.accessioned2020-12-14T05:57:37Z
dc.date.available2020-12-14T05:57:37Z
dc.date.issued2020-05
dc.identifierpii: 10.1038/s41565-020-0642-4
dc.identifier.citationSenders, M. L., Meerwaldt, A. E., van Leent, M. M. T., Sanchez-Gaytan, B. L., van de Voort, J. C., Toner, Y. C., Maier, A., Klein, E. D., Sullivan, N. A. T., Sofias, A. M., Groenen, H., Faries, C., Oosterwijk, R. S., van Leeuwen, E. M., Fay, F., Chepurko, E., Reiner, T., Duivenvoorden, R., Zangi, L. ,... Mulder, W. J. M. (2020). Probing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging.. Nat Nanotechnol, 15 (5), pp.398-405. https://doi.org/10.1038/s41565-020-0642-4.
dc.identifier.issn1748-3387
dc.identifier.urihttp://hdl.handle.net/11343/254061
dc.description.abstractIschaemic heart disease evokes a complex immune response. However, tools to track the systemic behaviour and dynamics of leukocytes non-invasively in vivo are lacking. Here, we present a multimodal hot-spot imaging approach using an innovative high-density lipoprotein-derived nanotracer with a perfluoro-crown ether payload (19F-HDL) to allow myeloid cell tracking by 19F magnetic resonance imaging. The 19F-HDL nanotracer can additionally be labelled with zirconium-89 and fluorophores to detect myeloid cells by in vivo positron emission tomography imaging and optical modalities, respectively. Using our nanotracer in atherosclerotic mice with myocardial infarction, we observed rapid myeloid cell egress from the spleen and bone marrow by in vivo 19F-HDL magnetic resonance imaging. Concurrently, using ex vivo techniques, we showed that circulating pro-inflammatory myeloid cells accumulated in atherosclerotic plaques and at the myocardial infarct site. Our multimodality imaging approach is a valuable addition to the immunology toolbox, enabling the study of complex myeloid cell behaviour dynamically.
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.titleProbing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging.
dc.typeJournal Article
dc.identifier.doi10.1038/s41565-020-0642-4
melbourne.affiliation.departmentMedicine (RMH)
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleNature Nanotechnology
melbourne.source.volume15
melbourne.source.issue5
melbourne.source.pages398-405
melbourne.elementsid1446263
melbourne.openaccess.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416336
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416336
melbourne.openaccess.statusAccepted version
melbourne.contributor.authorMaier, Andrea
dc.identifier.eissn1748-3395
melbourne.accessrightsAccess this item via the Open Access location


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