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    Tisagenlecleucel cellular kinetics, dose, and immunogenicity in relation to clinical factors in relapsed/refractory DLBCL

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    Author
    Awasthi, R; Pacaud, L; Waldron, E; Tam, CS; Jager, U; Borchmann, P; Jaglowski, S; Foley, SR; van Besien, K; Wagner-Johnston, ND; ...
    Date
    2020-02-11
    Source Title
    Blood Advances
    Publisher
    AMER SOC HEMATOLOGY
    University of Melbourne Author/s
    Tam, Constantine
    Affiliation
    Medicine and Radiology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Awasthi, R., Pacaud, L., Waldron, E., Tam, C. S., Jager, U., Borchmann, P., Jaglowski, S., Foley, S. R., van Besien, K., Wagner-Johnston, N. D., Kersten, M. J., Schuster, S. J., Salles, G., Maziarz, R. T., Anak, O., del Corral, C., Chu, J., Gershgorin, I., Pruteanu-Malinici, I. ,... Waller, E. K. (2020). Tisagenlecleucel cellular kinetics, dose, and immunogenicity in relation to clinical factors in relapsed/refractory DLBCL. BLOOD ADVANCES, 4 (3), pp.560-572. https://doi.org/10.1182/bloodadvances.2019000525.
    Access Status
    Access this item via the Open Access location
    URI
    http://hdl.handle.net/11343/254171
    DOI
    10.1182/bloodadvances.2019000525
    Open Access URL
    http://doi.org/10.1182/bloodadvances.2019000525
    Abstract
    The anti-CD19 chimeric antigen receptor (CAR)-T cell therapy tisagenlecleucel was evaluated in the global, phase 2 JULIET study in adult patients with relapsed/refractory diffuse large B-cell lymphoma (DLBCL). We correlated tisagenlecleucel cellular kinetics with clinical/product parameters in 111 patients treated in JULIET. Tisagenlecleucel persistence in responders and nonresponders, respectively, was demonstrated for 554 and 400 days maximum by flow cytometry and for 693 and 374 days maximum by quantitative polymerase chain reaction (qPCR). No relationships were identified between cellular kinetics (qPCR) and product characteristics, intrinsic/extrinsic factors, dose, or immunogenicity. Most patients with 3-month response had detectable transgene at time of response and continued persistence for ≥6 months. Expansion (maximal expansion of transgene/CAR-positive T-cell levels in vivo postinfusion [Cmax]) was potentially associated with response duration but this did not reach statistical significance (hazard ratio for a twofold increase in Cmax, 0.79; 95% confidence interval, 0.61-1.01). Tisagenlecleucel expansion was associated with cytokine-release syndrome (CRS) severity and tocilizumab use; no relationships were observed with neurologic events. Transgene levels were associated with B-cell levels. Dose was associated with CRS severity, but this was not statistically significant after adjusting for baseline tumor burden. In contrast to the results from B-cell precursor acute lymphoblastic leukemia (B-ALL) and chronic lymphocytic leukemia, similar exposure was observed in DLBCL in this study regardless of response and expansion was lower in DLBCL than B-ALL, likely from differences in cancer location and/or T-cell intrinsic factors. Relationships between expansion and CRS severity, and lack of relationships between dose and exposure, were similar between DLBCL and B-ALL. Tisagenlecleucel cellular kinetics in adult relapsed/refractory DLBCL improve current understanding of in vivo expansion and its relationships with safety/efficacy endpoints. This trial was registered at www.clinicaltrials.gov as #NCT02445248.

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