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    Rapid Gel Card Agglutination Assays for Serological Analysis Following SARS-CoV-2 Infection in Humans

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    Author
    Alves, D; Curvello, R; Henderson, E; Kesarwani, V; Walker, JA; Leguizamon, SC; McLiesh, H; Raghuwanshi, VS; Samadian, H; Wood, EM; ...
    Date
    2020-08-28
    Source Title
    ACS sensors
    Publisher
    AMER CHEMICAL SOC
    University of Melbourne Author/s
    Graham, Maryza
    Affiliation
    Microbiology and Immunology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Alves, D., Curvello, R., Henderson, E., Kesarwani, V., Walker, J. A., Leguizamon, S. C., McLiesh, H., Raghuwanshi, V. S., Samadian, H., Wood, E. M., McQuilten, Z. K., Graham, M., Wieringa, M., Korman, T. M., Scott, T. F., Holl, M. M. B., Garnier, G. & Corrie, S. R. (2020). Rapid Gel Card Agglutination Assays for Serological Analysis Following SARS-CoV-2 Infection in Humans. ACS SENSORS, 5 (8), pp.2596-2603. https://doi.org/10.1021/acssensors.0c01050.
    Access Status
    Access this item via the Open Access location
    URI
    http://hdl.handle.net/11343/254377
    DOI
    10.1021/acssensors.0c01050
    Open Access URL
    https://europepmc.org/articles/PMC7370531?pdf=render
    Abstract
    High-throughput and rapid serology assays to detect the antibody response specific to severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) in human blood samples are urgently required to improve our understanding of the effects of COVID-19 across the world. Short-term applications include rapid case identification and contact tracing to limit viral spread, while population screening to determine the extent of viral infection across communities is a longer-term need. Assays developed to address these needs should match the ASSURED criteria. We have identified agglutination tests based on the commonly employed blood typing methods as a viable option. These blood typing tests are employed in hospitals worldwide, are high-throughput, fast (10-30 min), and automated in most cases. Herein, we describe the application of agglutination assays to SARS-CoV-2 serology testing by combining column agglutination testing with peptide-antibody bioconjugates, which facilitate red cell cross-linking only in the presence of plasma containing antibodies against SARS-CoV-2. This simple, rapid, and easily scalable approach has immediate application in SARS-CoV-2 serological testing and is a useful platform for assay development beyond the COVID-19 pandemic.

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