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dc.contributor.authorAlves, D
dc.contributor.authorCurvello, R
dc.contributor.authorHenderson, E
dc.contributor.authorKesarwani, V
dc.contributor.authorWalker, JA
dc.contributor.authorLeguizamon, SC
dc.contributor.authorMcLiesh, H
dc.contributor.authorRaghuwanshi, VS
dc.contributor.authorSamadian, H
dc.contributor.authorWood, EM
dc.contributor.authorMcQuilten, ZK
dc.contributor.authorGraham, M
dc.contributor.authorWieringa, M
dc.contributor.authorKorman, TM
dc.contributor.authorScott, TF
dc.contributor.authorHoll, MMB
dc.contributor.authorGarnier, G
dc.contributor.authorCorrie, SR
dc.identifier.citationAlves, D., Curvello, R., Henderson, E., Kesarwani, V., Walker, J. A., Leguizamon, S. C., McLiesh, H., Raghuwanshi, V. S., Samadian, H., Wood, E. M., McQuilten, Z. K., Graham, M., Wieringa, M., Korman, T. M., Scott, T. F., Holl, M. M. B., Garnier, G. & Corrie, S. R. (2020). Rapid Gel Card Agglutination Assays for Serological Analysis Following SARS-CoV-2 Infection in Humans. ACS SENSORS, 5 (8), pp.2596-2603.
dc.description.abstractHigh-throughput and rapid serology assays to detect the antibody response specific to severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) in human blood samples are urgently required to improve our understanding of the effects of COVID-19 across the world. Short-term applications include rapid case identification and contact tracing to limit viral spread, while population screening to determine the extent of viral infection across communities is a longer-term need. Assays developed to address these needs should match the ASSURED criteria. We have identified agglutination tests based on the commonly employed blood typing methods as a viable option. These blood typing tests are employed in hospitals worldwide, are high-throughput, fast (10-30 min), and automated in most cases. Herein, we describe the application of agglutination assays to SARS-CoV-2 serology testing by combining column agglutination testing with peptide-antibody bioconjugates, which facilitate red cell cross-linking only in the presence of plasma containing antibodies against SARS-CoV-2. This simple, rapid, and easily scalable approach has immediate application in SARS-CoV-2 serological testing and is a useful platform for assay development beyond the COVID-19 pandemic.
dc.titleRapid Gel Card Agglutination Assays for Serological Analysis Following SARS-CoV-2 Infection in Humans
dc.typeJournal Article
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.source.titleACS Sensors
melbourne.openaccess.statusPublished version
melbourne.contributor.authorGraham, Maryza
melbourne.accessrightsAccess this item via the Open Access location

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