University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Microbiology & Immunology
  • Microbiology & Immunology - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Melbourne Medical School
  • Microbiology & Immunology
  • Microbiology & Immunology - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir.

    Thumbnail
    Citations
    Scopus
    Web of Science
    Altmetric
    1
    1
    Author
    Bokelmann, M; Edenborough, K; Hetzelt, N; Kreher, P; Lander, A; Nitsche, A; Vogel, U; Feldmann, H; Couacy-Hymann, E; Kurth, A
    Date
    2020-01
    Source Title
    PLoS Neglected Tropical Diseases
    Publisher
    Public Library of Science (PLoS)
    University of Melbourne Author/s
    Edenborough, Kathryn
    Affiliation
    Microbiology and Immunology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Bokelmann, M., Edenborough, K., Hetzelt, N., Kreher, P., Lander, A., Nitsche, A., Vogel, U., Feldmann, H., Couacy-Hymann, E. & Kurth, A. (2020). Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir.. PLoS Negl Trop Dis, 14 (1), pp.e0007952-. https://doi.org/10.1371/journal.pntd.0007952.
    Access Status
    Access this item via the Open Access location
    URI
    http://hdl.handle.net/11343/254408
    DOI
    10.1371/journal.pntd.0007952
    Open Access URL
    https://europepmc.org/articles/PMC6994141?pdf=render
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994141
    Abstract
    The significance of the integral membrane protein Niemann-Pick C1 (NPC1) in the ebolavirus entry process has been determined using various cell lines derived from humans, non-human primates and fruit bats. Fruit bats have long been purported as the potential reservoir host for ebolaviruses, however several studies provide evidence that Mops condylurus, an insectivorous microbat, is also an ebolavirus reservoir. NPC1 receptor expression in the context of ebolavirus replication in microbat cells remains unstudied. In order to study Ebola virus (EBOV) cellular entry and replication in M. condylurus, we derived primary and immortalized cell cultures from 12 different organs. The NPC1 receptor expression was characterized by confocal microscopy and flow cytometry comparing the expression levels of M. condylurus primary and immortalized cells, HeLa cells, human embryonic kidney cells and cells from a European microbat species. EBOV replication kinetics was studied for four representative cell cultures using qRT-PCR. The aim was to elucidate the suitability of primary and immortalized cells from different tissues for studying NPC1 receptor expression levels and their potential influence on EBOV replication. The NPC1 receptor expression level in M. condylurus primary cells differed depending on the organ they were derived from and was for most cell types significantly lower than in human cell lines. Immortalized cells showed for most cell types higher expression levels than their corresponding primary cells. Concluding from our infection experiments with EBOV we suggest a potential correlation between NPC1 receptor expression level and virus replication rate in vitro.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [53102]
    • Microbiology & Immunology - Research Publications [2207]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors