Clinical evaluation of AusDiagnostics SARS-CoV-2 multiplex tandem PCR assay
AuthorAttwood, LO; Francis, MJ; Hamblin, J; Korman, TM; Druce, J; Graham, M
Source TitleJournal of Clinical Virology
AffiliationMicrobiology and Immunology
Document TypeJournal Article
CitationsAttwood, L. O., Francis, M. J., Hamblin, J., Korman, T. M., Druce, J. & Graham, M. (2020). Clinical evaluation of AusDiagnostics SARS-CoV-2 multiplex tandem PCR assay. JOURNAL OF CLINICAL VIROLOGY, 128, https://doi.org/10.1016/j.jcv.2020.104448.
Access StatusAccess this item via the Open Access location
Open Access URLhttps://europepmc.org/articles/PMC7236671?pdf=render
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236671
BACKGROUND: In the context of the pandemic, the rapid emergency use authorisation of diagnostic assays for SARS-CoV-2 has meant there are few peer-reviewed published studies of clinical performance of commercial assays. AIMS: To evaluate the clinical performance of AusDiagnostics respiratory multiplex tandem PCR assay including SARS-CoV-2. METHODS: We reviewed the results following implementation of AusDiagnostics respiratory multiplex tandem PCR assay including SARS-CoV-2, and compared with an in-house RT-PCR assay at our State Reference Laboratory. RESULTS: Initial validation using AusDiagnostics coronavirus multiplex tandem PCR assay including SARS-CoV-2 demonstrated good concordance with the State Reference Laboratory. After implementing the AusDiagnostics respiratory multiplex tandem PCR assay including SARS-CoV-2, we tested 7839 samples. 127 samples in which SARS-CoV-2 was detected using the AusDiagnostics assay were referred for testing at the State Reference Laboratory, with concordant results in 118/127 (92.9%) of samples. After resolution of discrepancies, 125/127 (98.4%) of AusDiagnostics results were determined to be true positive results. Out of 7839 samples tested for SARS-CoV-2 during this period, only 2 tests (0.02%) were indeterminate results. CONCLUSION: The AusDiagnostics respiratory MT-PCR assay is a reliable assay for detection of SARS-CoV-2.
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