Show simple item record

dc.contributor.authorCaly, L
dc.contributor.authorDruce, J
dc.contributor.authorRoberts, J
dc.contributor.authorBond, K
dc.contributor.authorTran, T
dc.contributor.authorKostecki, R
dc.contributor.authorYoga, Y
dc.contributor.authorNaughton, W
dc.contributor.authorTaiaroa, G
dc.contributor.authorSeemann, T
dc.contributor.authorSchultz, MB
dc.contributor.authorHowden, BP
dc.contributor.authorKorman, TM
dc.contributor.authorLewin, SR
dc.contributor.authorWilliamson, DA
dc.contributor.authorCatton, MG
dc.date.accessioned2020-12-16T23:39:35Z
dc.date.available2020-12-16T23:39:35Z
dc.date.issued2020-04-01
dc.identifier.citationCaly, L., Druce, J., Roberts, J., Bond, K., Tran, T., Kostecki, R., Yoga, Y., Naughton, W., Taiaroa, G., Seemann, T., Schultz, M. B., Howden, B. P., Korman, T. M., Lewin, S. R., Williamson, D. A. & Catton, M. G. (2020). Isolation and rapid sharing of the 2019 novel coronavirus (SAR-CoV-2) from the first patient diagnosed with COVID-19 in Australia. MEDICAL JOURNAL OF AUSTRALIA, 212 (10), pp.459-462. https://doi.org/10.5694/mja2.50569.
dc.identifier.issn0025-729X
dc.identifier.urihttp://hdl.handle.net/11343/254447
dc.description.abstractOBJECTIVES: To describe the first isolation and sequencing of SARS-CoV-2 in Australia and rapid sharing of the isolate. SETTING: SARS-CoV-2 was isolated from a 58-year-old man from Wuhan, China who arrived in Melbourne on 19 January 2020 and was admitted to the Monash Medical Centre, Melbourne from the emergency department on 24 January 2020 with fever, cough, and progressive dyspnoea. MAJOR OUTCOMES: Clinical course and laboratory features of the first reported case of COVID-19 (the illness caused by SARS-CoV-2) in Australia; isolation, whole genome sequencing, imaging, and rapid sharing of virus from the patient. RESULTS: A nasopharyngeal swab and sputum collected when the patient presented to hospital were each positive for SARS-CoV-2 (reverse transcription polymerase chain reaction). Inoculation of Vero/hSLAM cells with material from the nasopharyngeal swab led to the isolation of SARS-CoV-2 virus in culture. Electron microscopy of the supernatant confirmed the presence of virus particles with morphology characteristic of viruses of the family Coronaviridae. Whole genome sequencing of the viral isolate and phylogenetic analysis indicated the isolate exhibited greater than 99.99% sequence identity with other publicly available SARS-CoV-2 genomes. Within 24 hours of isolation, the first Australian SARS-CoV-2 isolate was shared with local and overseas reference laboratories and major North American and European culture collections. CONCLUSIONS: The ability to rapidly identify, propagate, and internationally share our SARS-CoV-2 isolate is an important step in collaborative scientific efforts to deal effectively with this international public health emergency by developing better diagnostic procedures, vaccine candidates, and antiviral agents.
dc.languageEnglish
dc.publisherWILEY
dc.titleIsolation and rapid sharing of the 2019 novel coronavirus (SAR-CoV-2) from the first patient diagnosed with COVID-19 in Australia
dc.typeJournal Article
dc.identifier.doi10.5694/mja2.50569
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.departmentDoherty Institute
melbourne.source.titleMedical Journal of Australia
melbourne.source.volume212
melbourne.source.issue10
melbourne.source.pages459-462
melbourne.elementsid1444072
melbourne.openaccess.urlhttps://europepmc.org/articles/PMC7228321?pdf=render
melbourne.openaccess.statusPublished version
melbourne.contributor.authorTaiaroa, George
melbourne.contributor.authorWilliamson, Deborah
melbourne.contributor.authorLewin, Sharon
melbourne.contributor.authorSchultz, Mark
melbourne.contributor.authorSeemann, Torsten
melbourne.contributor.authorCaly, Leon
melbourne.contributor.authorHowden, Benjamin
melbourne.contributor.authorHowden, Brian
melbourne.contributor.authorDruce, Julian
melbourne.contributor.authorCatton, Michael
melbourne.contributor.authorBond, Katherine
melbourne.contributor.authorTran, Thomas
dc.identifier.eissn1326-5377
melbourne.accessrightsAccess this item via the Open Access location


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record